Implications of Auxins in Induction of Adventitious Roots from Leaf Explants of Cannon Ball Tree (Couroupita guianensis Aubl.)

ABSTRACT
An effective protocol has been developed for the adventitious root culture of Cannon Ball tree
(Couroupita guianensis Aubl.) from the leaves of in vitro germinated 30 days’ old seedlings. Seeds
germinated on cytokinins and auxins supplemented with Murashige and Skoog (MS) medium. Half
strength MS medium supplemented with 2.0 mg/L indole-3-butyric acid (IBA) was found the most
suitable for the induction of adventitious roots (92%) from the midrib region of the leaves than the
other auxins. The roots were proliferated on half strength MS liquid medium supplemented with IBA
0.5 mg/L. After eight weeks of inoculation the roots in the medium yielded 5.933 ±0.135 gm fresh and
2.731 ±0.010 gm dry biomass of adventitious roots. The growth rate was increased by 8-9 folds in this
investigation.

References
[1] M. Mitre M. 2012, http://www.iucnredlist.org.
[2] Y. Rai, International Journal of Innovative Science Research 8 (2014) 252-255.
[3] T. K. Lim. Springer Verlag, Berlin Heidelberg, 2012.
[4] K. C. Sai, N. Gaddala, S. Vanamala, V. Naresh, A. Elumalai, International Research
Journal of Pharmaceutical and Applied Science 1 (2011) 105-108.
[5] G. N. Shah, S. A. Shete, V. S. Patil, K. D. Patil, S. G. Killedar, International Journal of
Pharmacognosy and Phytochemical Research 4 (2012) 85-89.
[6] S. A. Shete, G. N. Shah, S. S. Walke, V. S. Patil, K. D. Patil, S. G. Killedar,
International Journal of Biology 2 (2013) 360-364.
[7] S. K. Gousia, K. K. Ashok, K. T. Vinay, L. L. Naveena, International Journal of
Pharmacy and Pharmaceutical Science Research 3 (2013) 140-143.
[8] B. Muniswamy, H. L. Sreenath, Indian Journal of Forestry 23 (2000) 202-204.
[9] C. Ramalakshmi, A. J. A. Ranjitsingh, K. Kalirajan, A. Kalirajan, G. Athinarayanan, R.
Mariselvam. Elixir Applied Biology 57 (2013) 14055-14057.
[10] A. A. Eknat, L. K. Shivchandraji, Indian Drugs 39 (2002) 213-216.
[11] R. P. Rastogi, B. N. Mehrotra, Compendium of Indian Medicinal Plants, CDRI
Lucknow, 4 (1995) 225.
[12] J. Bergman, J. Lindstrom, U. Tilstam, Tetrahedron 41 (1985) 2879-2881.
[13] H. Azimi, M. Fallah-Tafti, A. A. Khakshur, M. Abdollahi, Fitoterapia 83 (2012) 1306-
1317.
[14] A. R. Bafna, S. H. Mishra, R. S. Deoda, P. A. Bafna, R. H. Kale, International Journal
of Pharmacy Pharmaceutical Science Research 3 (2011) 4-6.
[15] S. S. Wankhede, M. Gambhire, A. Juvekar, N. P. Marg, Pharmacologyonline 2 (2009)
999-1013.
[16] A. Elumalai, M. C. Eswaraiah, A. Didala, International Journal of Pharmaceutical
Science 3 (2012) 2262-2269.
[17] M. Geetha, A. K. Saluja, M. B. Shankar, R. S. Mehta, Journal of Natural Remedies 4
(2004) 52-55.
[18] M. Geetha, M. B. Shankar, R. S. Mehta, A. K. Saluja, Journal of Natural Remedies 5
(2005) 121-125.
[19] N. A. Al-dhabi, C. Balachandran, M. K. Raj, V. Duraipandiyan, C. Muthukumar, S.
Ignacimuthu, V. S. Rajput, BMC Complementary and Alternative Medicine 12 (2012)
242.
[20] D. Pradhan, P. K. Panda, G. Tripathy, Naural Product Radiance 8 (2009) 37-42.
[21] M. R. M. Usman, R. P. Somani, A. Mohammed, U. Mohammed, Research Journal of
Pharmaceutical, Biological and Chemical Science 3 (2012) 825-834.
[22] H. G. Vinod, A. G. Mahendra S. W. Shaijesh, S. D. Vishal, R. J. Archana, International
Journal of Pharmacy and Phytopharmacological Research 1 (2012) 242-246.
[23] S. P. Umachigi, K. N. Jayaveera, C. K. Ashok, G. S. Kumar, Pharmacology online 281
(2007) 269-281.
[24] M. M. Pinheiro, S. O. Bessa, C. E. Fingolo, R. M. Kuster, M. E. Matheus, F. S.
Menezes, P. D. Fernandez, Journal of Ethnopharmacology 127 (2010) 407-413.
[25] A. Elumalai, M. C. Eswaraiah, K. K. Naresh, R. Kumar, A. Meruva, C. Vidhyulatha,
International Journal of Pharmacology 3 (2013) 42-44.
[26] K. Baskar, S. Ignacimuthu, Archives of Phytopathology and Plant Protection 46 (2013)
1571-1579.
[27] S. Lingathurai, S. E. Vendan, M. G. Paulraj, S. Ignacimuthu, Journal of King Saud
University-Science 23 (2011) 11-16.
[28] S. G. Golatkar, V. R. Kamath, J. B. Rane, S. J. Vahanwala, Indian Drugs 38 (2001)
102-103.
[29] J. B. Sanz, J. Campos-de-la-Cruz, M. A. Epiquién-Rivera, S. Canigueral, S Journal of
Ethnopharmacology 122 (2009) 333-362.
[30] R. S. Rao,G. A. Ravishankar, Biotechnology Advances 20 (2002) 101-153.
[31] G. Sivanandhan, M. Arun, S. Mayavan, M. Rajesh, T. S. Mariashibu, M.
Manickavasagam, N. Selvaraj, A. Ganapathi, Industrial crops and products 37 (2012)
124-129.
[32] H. R. Deepa. The Hindu, Chennnai, Tamil Nadu, India, 2007.
[33] H. C. Chaturvedi, M. Sharma, In proc. Contedanbs Symp.(ed. Roa, A. N.), 1981,
Singapore, p. 31-302.
[34] V. Mulabagal, H. S. Tsay, International Journal of Engineering and Applied Science 1
(2004) 29-48.
[35] H. P. Bais, V. M. L. Vargas, H. E. Flores, J. M. Vivanco, In Vitro Cell and
Developmental Bioogy-Plant 37 (2001) 730-741.
[36] T. B. Fulzele, R. K. Satdive, B. B. Pol, Plant Cell Tissue and Organ Culture 69 (2002)
285-288.
[37] H. N. Murthy, C. Dijkstra, P. Anthony, D. A. White, M. R. Davey, J. B. Powers, E. J.
Hahn, K. Y. Paek, Journal of Integrated Plant Biology 50 (2008) 915-981.
[38] S. Q. Wu, M. L. Lian, R. Gao, S.Y. Park, X.C. Piao, In Vitro Cell and Developmental
Biology 47 (2011) 719-724.
[39] R. V. Reis, A.P.P.L. Borges, T.P.C. Chierrito, E.R. de Souto and L.M. de Souza, Plant
Cell Tissue and Organ Culture 106 (2011) 329-335.
[40] M. Z. Saiman, N. R. Mustafa, A. E. Schulte, R. Verpoorte, Y. H. Choi, Plant Cell
Tissue and Organ Culture 2012, doi 10.1007/s11240-011-0111-8.
[41] H. Y. Cui, A. Baque, E.J. Lee, K.Y. Paek, Plant Biotechnology Reports 7 (2013) 297-
308.
[42] J. Wang, W. Gao, Q. Wang, S. Man, L. Zhang, Research in Chemical Intermediates 41
(2013) 623-629.
[43] M. Rajesh, G. Sivanandhan, M. Arun, V. Vasudevan, J. Theboral, Acta Physiologiae
Plantarum 36 (2014) 1009-1021.
[44] S. Yin, Y. Zhang, W. Gao, J. Wang, S. Man, H. Liu, Acta Physiologiae Plantarum 36
(2014) 915-921.
[45] C. Sorin, J. D. Bussell, I. Camus, K. Ljung, M. Kowalczyk, G. Geiss, H. Mckhann, C.
Garcion, H. Vaucheret, G. Sandberg, Plant Cell 17 (2005) 1343-1359.
[46] N. Praveen, S. H. Manohar, P. M. Naik, A. Nayeem, J. H. Jeong, H. N. Murthy, Current
Science 96 (2009) 5-10.
[47] T. Murashige, F. A. Skoog, Physiologia Plantarum 15 (1962) 473-497.
[48] G. Sivakumar, K. W. Yu, K. Y. Paek, Life Science 5 (2005) 4.
[49] S. Ahmed, E. J. Hahn, K. Y. Paek, Journal of Plant Biology 51 (2008) 209-212.
[50] D. L. Pacurar, I. Perrone, C. Bellini, Physiologia Plantarum (2014). Doi.
10.1111/11ppl.12171.
[51] P. Baskaran, N. Jayabalan, Biotechnology Letter 31 (2009) 1073-1077.
[52] Y. K. Yang, S. Y. Lee, W. T. Park, N. I. Park, S. U. Park. Plant Omics Journal 3 (2010)
190-193.
[53] A. Pandey, S. Tamta, D. Giri, Internationl Journal of Biodiversity and Conservation 3
(2011) 142-146.
[54] G. Mahendranath, A. Venugopalan, R. Parimalan, P. Giridhar, G. A. Ravishankar, Plant
Cell Tissue and Organ Culture 106 (2011) 517-522.
[55] A.P. K. Ling, K. P. Tan, S. Hussein, Journal of Zhejiang University-Science B
(Biomedicine & Biotechnology) 14 (2013) 621-631.
[56] C. Umamaheswari, A. Ambethkar, F. S. Margater, N. Selvaraj, International Journal of
Innovative Research in Science, Engineering and Technology 3 (2014) 16886-16895.
[57] S. Nandagopal, B. D. R. Kumari, Journal of Central European Agriculture 8 (2007) 73-80.
[58] M. A. Baque, A. Elgirban, E. J. Lee, K. Y. Paek, Acta Physiologia Plantarum 34 (2012)
405-415.
[59] Z. Zhang, Z. Yu, Z. Jin, J. Liu, Y. Li, Advance Journal of Food Science and Technology
5 (2013) 127-131.

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WSN 33 (2016) 109-121