ABSTRACT
The most dominant external and internal factors in influencing fish gonad maturation are temperature and feed. Temperature can accelerate metabolism and protein synthesis which causes gonad maturity. Feed with high protein is needed in the process of gamete formation. The purpose of this study was to determine the use of optimum temperature and mixed feed (salted-boiled kawakawa tuna (Euthynnus affinis)+commercial feed) to the value of the Gonadosomatic Index (GSI) and Feed Conversion Ratio (FCR). The method used in this research was an experimental method with a completely randomized design (CRD), which consisted of 4 treatments and 3 replications. The use of optimum temperature 30 ± 1⁰C, and four feed with the addition of salted-boiled kawakawa tuna (Euthynnus affinis) as much as 3.5%, 2.5%, 1.5%, and 3.5% on non-transgenic catfish with 56 days of rearing. A comparison of gonad weight and fish body weight was observed to calculate the GSI value while the ratio of the amount of feed given was observed to calculate the FCR value. The highest Gonadosomatic Index (GSI) value was found in fish with the addition of 2,5% salted-boiled kawakawa tuna (Euthynnus affinis) produced a GSI value of the female broodstock of 35.510 ± 0.738%, and the male broodstock produced a GSI value of 3.555 ± 0.241%. Feed Conversion Ratio (FCR) in G4 transgenic Mutiara catfish with treatment A (3.5%), treatment B (2.5%), treatment C (1.5%), and treatment D (control) were respectively (1.002±0.405), (0.559±0.026), (0.765±0.405), and (3.350±0.856). Transgenic Mutiara catfish G4 fed 2,5% salted-boiled kawakawa tuna (Euthynnus affinis) had the best feed conversion ratio (FCR) compared to non-transgenic Mutiara catfish.
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