ABSTRACT
The insects are a class of invertebrates within the arthropod phylum that have a chitinous
exoskeleton. The leaf eating insects obtain their nutrients and growth promoting biocompounds from
the variable or specific flora available for them. The plants on earth are the richest source of
metabolites including juvenile hormone analogues for leaf eating insects like silkworm, Bombyx mori
(L). Some of plant origin metabolites are acting as insects juvenoids for insect lives. They serve to
take pause in the progression of metamorphosis through arresting some of the biochemical reactions
including chitin synthesis or accelerating progression through other biochemical pathways in the larval
body of insects. The ten microliters of various concentrations of acetone solution of Fernasol Methyl
Ether (FME) and each selected monoterpene compounds (Myrcene; Camphene; Cymene; Limonene
and Eucalyptol ) were used for topical application to individual larval instars of silkworm, Bombyx
mori (L) (Race: PM x CSR2) at 48 hours after the fourth moult. The integument chitin of untreated
control larvae; acetone treated control; FME treated larvae and monoterpene treated larvae was
estimated at 120 hours after the fourth moult. Topical application of selected concentrations of acetone
solutions of selected monoterpenes to fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x
CSR2) was found reflected into the reduction in the deposition of chitin in the larval body wall. The
reduction in body wall chitin was found ranging from zero to hundred percent. The plot of
concentrations of acetone solutions of FME and monoterepene compounds and percent reduction in
the body wall chitin was found exhibiting a characteristic Sigmoid form of displacement, which
herewith titled as “Punyamayee Baramati Dose Response Curve”. Since the effects of juvenoids
involve the inhibition of metamorphosis through reduction in chitin deposition, it is possible to express
the concentration (dose) applied in terms of ID50 value. The ID50 value of juvenoid contents of FME
and selected monoterpene compounds can be defined as the specific unit (microgram), which enable to
chitin to deposit fifty percent less in the body wall of larvae (In comparison with untreated control).
Accordingly, the ID50 value calculated from the “Punyamayee Baramati Dose Response Curves” for
FME was found measured 0.08 mg/ml. The ID50 values for monoterpene compounds: Myrcene;
Camphene; Cymene; Limonene and Eucalyptol were found measured: 0.116; 0.122; 0.164; 0.172 and
0.208 mg/ml respectively. Acetone soluble juvenoid content of terpene compounds may be utilized
efficiently for the fortified development of fifth instars of silkworm, Bombyx mori (L) and thereby, the
cocoon quality. Sigmoid (S-form) “Baramati Dose Response Curve” may help for quantitative
estimation of juvenoid contents of various terpene compounds and terpenoids.
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