Phytochemical and Essential Oil Quantification of the Aerial Parts of Commelina diffusa

ABSTRACT

There are numerous medicinal plants in Southern and Eastern Nigeria, which are widely exploited in the Nigerian traditional system of medicine for the treatment of countless of illnesses. These plants are very rich in bioactive source of drugs, modern medicines, nutraceuticals, food supplements, pharmaceutical intermediates, and chemical entities for synthetic drugs, which has not been characterized. Commelina diffusa is widely utilized in folklore medicine for the treatment of several bacterial infections. This paper focused on the phytochemical and essential quantification of the aerial parts of Commelina diffusa. Phytochemical and essential oil quantification were carried out using high performance liquid chromatography and gas chromatography. Analysis of the aerial parts of Commelina diffusa for phytochemicals revealed the presence of eleven flavonoids with a total 133.41 mg/100g, seven terpenoids (45.72 mg/100g), nine phenolic acids (199.76 mg/100g), seven cyanogenic glycoside (194.99 mg/100g), twelve glycosides (128.57 mg/100g), fourteen alkaloids (35.48 mg/100g), fourteen lignans (265.7 mg/100g), eighteen saponins (82.9 mg/100g), twelve anthocyanins (57.22 mg/100g), thirteen anthraquinones (168.66 mg/100g), thirteen sterols (45.67 mg/100g), and essential oils (100%). Our unearthing  recommends Commelina diffusa is a magnificent birthplace for essential oils, terpenoids, saponins, alkaloids, anthraquinones, anthocyanins, phenolic acid, sterols, cyanogenic glycoside, glycosides, and flavonoids. This investigation personify and demonstrate the rudimentary identification for tactfulness or nomination of Commelina diffusa prospective source of novel therapies for the treatment of multitudinous diseases.

References

• Edeoga, H.O., Okwu, D., and Mbaebie, B.O. 2005. Phytochemical constituents of some Nigerian Medicinal plants. J. Biotechnol, 4(7): 685-688
• Akharaiyi, F.C., and Boboye, B. 2010. Antibacterial and phytochemical evaluation of three medicinal plants, J Nat Prod, 3: 27-34
• Rout, S.P., Choudhury, K.A., Kar, D.M., Das, L., and Jain, A. 2009. Plants in traditional medicinal system-future source of new drugs. J. Pharmacy Pharmaceurical. Sci, 1(1): 1-23
• Wellington, E.O, Peters, D. E, and Onyeike, E.N. Chemical composition of phytochemicals and essential oil of Euphorbia heterophylla. International Journal of Advanced Research and Publications, 3: 11.
• Lingaraju, K., Raja, N., Nagabhushana, G and Nagaraju, G. 2019. Euphorbia heterophylla (L.) mediated fabrication of ZnO NPs: Characterization and evaluation of antibacterial and anticancer properties. Biocatalysis and Agricultural Biotechnology, 18, 100894
• Ball, S., Arevalo, M., Juarez, E., Payne, J.D., and Jones, C. 2019. Breast cancer chemoprevention: An update on current practice and opportunities for primary care physicians. Preventive Medicine, 129: 105834
• Oyagbemi, A.A., Saba, A.B., and Azeez, O.I. 2010. Capsaicin: A novel chemopreventive molecule and its underlying molecular mechanisms of action. Indian J. Cancer, 47: 53–58
• Venier,A., Colquhoun, A.J., Sasaki, H., Kiss, A., Sugar, L., Adomat, H., Fleshner, N.E., Klotz, L.H., and Venkateswaran, V. 2015. Capsaicin: A novel radio-sensitizing agent for prostate cancer. Prostate, 75: 113–125.
• Chapa-Oliver, A.M., and Mejia-Teniente, L. 2016. Capsaicin: From Plants to a Cancer-Suppressing Agent. Molecules, 21: 931
• Wellington, E. O., Onyeike, E. N., Peters, D. E., Joshua, D., and Ogbomade, S. J. 2022. Phytochemical and Essential Oil Characterization of the Aerial Parts of Leonurus cardiaca (Motherworth). Asian Journal of Applied Chemistry Research, 11(1): 55-72
• Jose-Luis, R. 2016. Essential Oils in Food Preservation, Flavor and Safety, 3-10
• Asgari,B., Ghorbanpour, M., and Nikabadi, S. 2017. Heavy metals in contaminated environment: Destiny of secondary metabolite biosynthesis, oxidative status and phytoextraction in medicinal plants. Ecotoxicology and Environmental Safety, 145: 377–390
• Amirifar, A., Hemati, A., Asgari, Lajayer, B., Pandey, J., and Astatkie, T. 2022. Impact of various environmental factors on the biosynthesis of alkaloids in medicinal plants. In: Aftab T., editor. Environmental Challenges and Medicinal Plants. Springer; Cham, Switzerland: 229–248
• Khan, M.A.A., Islam, M.T. and Sadhu, S.K. 2011. Evaluation of Phytochemical and Antimicrobial Properties of Commelina diffusa. Oriental Pharmacy and Experimental Medicine, 11, 235-241
• Mensah, A.Y., Houghton, P.J., Dickson, R.A., Fleischer, T.C., Heinrich, M. and Bremner, P. 2006. In Vitro Evaluation of Effects of Two Ghanaian Plants Relevant to Wound Healing. Phytotherapy Research, 20, 941-944
• Mensah,Y., Mireku, E.A., Damoah, A.O. and Amponsah, I.K. 2014. Anti-Inflammatory and Antioxidant Activities of Commelina diffusa (Commelinaceae). World Journal of Pharmaceutical Sciences, 2, 1159-1165.
• Alim, A., Goze, I., Goze, H.M, and Tepe, B. 2009. In vitro antimicrobial and antiviral activities of the essential oil and various extracts of Salvia cedronella Boiss. J Med Plant Res, 3(5): 413-419
• Chemat, F, Albet, M, and Fernandez, X. 2012. Microwave-asssted extraction of Terpenoid and Essential oils. Microwave-assisted Extraction of Bioactive Compounds Springer, 12: 53-68
• Rahman, G., Syed, U.J., Syed, F., Samiullah, S., and Nusrat, J. 2017. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan. Scientific World Journal, 4(12): 27-39
• Vitaglione, P, Donnarumma, G, and Napolitano, A. 2007. Protocatechuic acid is the major human metabolite of cyanidin-glucosides. The Journal of Nutrition, 137(9):2043–2048.
• Perry,K., Tomlinson, B.E., and Blessed, G, 1978. Correlation of cholinergic abnormalities with senile plaques and mental test scores in senile dementia. Br Med J 2, 1457–1459
• Smith, R., DeWitt, D and Garavito, R. 2000. Cyclooxygenases: structural, cellular and molecular biology. Ann Rev Biochem 69: 145–182
• Kurumbail,, Stevens, A, and Gierse, J. 1996. Structural basis for selective inhibition of cyclooxygenase 2 by anti-inflammatory agents. Nature, 384: 644–648
• Madeswaran, A., Umamaheswari, M., and Asokkumar, K. 2012. In-silico docking studies of cyclooxygenase inhibitory activity of commercially available flavonoids. Asian J Pharm Life Sci, 2: 174–181
• Nirmal, J., Sadanand, A. D., and Prahlad, P. 2019. Therapeutic and Medicinal Uses of Terpenes. Medicinal Plant, 12: 333–359
• Perumal, S. K., Madepalli, B., Gowdu, V., Muthanppan, V., and Kedike, B. 2017. Bauerenol, a triterpenoid from Indian Suregada angustifolia: Induces reactive oxygen species-mediated P38MAPK activation and apoptosis in human hepatocellular carcinoma (HepG2) cells. Tumout Biol, 39(4): 1010428317698387
• Barros ,F.W.A., Bandeira, P.N., Lima, D.J.B., Meira, A.S., de Farias, S.S., Albuquerque, M.R.J.R., dos Santos, H.S., Lemos, T.L.G., de Morais, M.O., and Costa-Lotufo, L.V. Amyrin esters induce cell death by apoptosis in HL-60 leukemia cells. Bioorg. Med. Chem, 19: 1268–1276
• Lima, E.M., Nascimento, A.M., Lenz, D., Scherer, R., Meyrelles, S.S., Boëchat, G.A.P., Andrade, T.U., and Endringer, D.C. 2014. Triterpenes from the Protium heptaphyllum resin-chemical composition and cytotoxicity. Bras. Farmacogn, 24: 399–407
• Oliveira,A., Vieira-Júnior, G.M., Chaves, M.H., Almeida, F.R.C., Florêncio, M.G., Lima, R.C.P., Silva, R.M., Santos, F.A., and Rao, V.S.N. 2014. Gastroprotective and anti-inflammatory effects of resin from Protium paraphylum in mice and rats. Pharmacal. Res, 49: 105–111
• Naresh, K., and Nidhi, G. 2019. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep (Amst), 24: e00370
• Kamila, Z., Agnieska, D., Anna, K., and Helena, R. 2018. Antioxidant Properties of Ferulic Acid and Its Possible Application. Skin Pharmacol Physiol, 31(6): 332-336.
• Min, Y., Chao, W., Tianxi, Z,. Lei, S., Jian, L., Hongbao, L., Xuzhen, L., Fengtang, J., Lu, Q., Tianlun, Z., Chenixi, W., Guangxu, L., Shuai, F., and Feng, L. 2022. Chicoric Acid: Natural Occurrence, Chemical Synthesis, Biosynthesis, and Their Bioactive Effects. Front Chem, 10: 888673
• Deniz, B., Zulfiye, G., Julie, A.M., Betul, C., Nilufer, C., and Mine, S.G. 2020. Pharmacologic Overview of Chlorogenic Acid and its Metabolites in Chronic Pain and Inflammation. Curr Neuropharmacol, 18(3): 216–228
• Zheng, Z., Sheng, Y., Lu, B., and Ji, L. 2015. The therapeutic detoxification of chlorogenic acid against acetaminophen-induced liver injury by ameliorating hepatic inflammation. Biol. Interact, 238: 93–101
• Pavle, R., Slavimir, V., Nedad, S., and Nebolisa, R. 2015. The Beneficial Biological Properties of Salicylic Acid. Interact, 32(4): 259-265
• Alaattin, S., Pelin, A., Gulsum, T., and Sevki, A. 2013. Anticarcinogenic Effect and Carcinogenic Potential of the Dietary Phenolic Acid: o-Coumaric Acid. Natural Product Communications, 8(9): 1269-1274
• Yoswaris, S., Patchareewan, P., Siriporn, C.C., and Nipon, C. 2015. Pharmacological Properties of Protocatechuic Acid and Its Potential Roles as Complementary Medicine. Molecule, 2015: 593902
• Xu-Na,, Kui-Yang,W., Xue-Song,Z., Chao,Y., and Xue-Ying,L. 2018. 4-Hydroxybenzoic acid (4-HBA) enhances the sensitivity of human breast cancer cells to adriamycin as a specific HDAC6 inhibitor by promoting HIPK2/p53 pathway. Biochemical and Biophysical Research Communications, 504 (4): 812-819
• Nielsen, L.J., Stuart, P., Pičmanová, M., Rasmussen, S., Olsen, C. E., Harholt, J., and Bjarnholt, N. 2016. Dhurrin metabolism in the developing grain of Sorghum bicolor (L.) Moench investigated by metabolite profiling and novel clustering analyses of time-resolved transcriptomic data. BMC Genomics, 17(1)
• Mosayyebi, B., Imani, M., Mohammadi, L., Akbarzadeh, A., Zarghami, N., Edalati, M., and Rahmati, M. 2020. An update on the toxicity of cyanogenic glycosides bioactive compounds: Possible clinical application in targeted cancer therapy. Materials Chemistry and Physics, 246, 122841
• Mhawesh, A., Al-Ugaili, D., and Olaiwy, Y. 2018. Investigate the antibacterial activity of amygdalin against some types of antibiotic resistant pathogenic bacteria. Basrah Journal of Veterinary Research, 17(3), 575–592
• Nwankwo, I. U., Onwuakor, C. E., and Nwosu, V. C. 2014. Phytochemical analysis and antibacterial activities of Citrullus lanatus seed against some pathogenic microorganisms. Global Journal of Medical Research, 14(4), 0975-5888.
• Nadayag, J., Dapar, M. L. G., and Aranas, A.T. 2019. Qualitative assessment
  of the antimicrobial, antioxidant, and phytochemical properties of the ethanolic
  extracts of the inner bark of Atuna racemosa. Pharmacophore, 10(1), 52–59
• Abtahi, H., Ghazavi, A., Karimi, M., Mollaghase, S., and Mosayebi, G. 2008. Antimicrobial Activities of Water and Methanol Extracts of Bitter Apricot Seeds. Journal of Medical Sciences, 8(4), 433-436
• Martinez Ruiz, M. G., Richard-Greenblatt, M., and Juarez, Z. N. 2012. Antimicrobial, Anti-Inflammatory, Antiparasitic, and Cytotoxic Activities of Laennecia confusa. Scientific World Journal, Article Number, 263572
• Langenhan, J.M., Peters, N.R., Guzei, I.A., Hoffmann, F.M. 2005. Thorson JS. Enhancing the anticancer properties of cardiac glycosides by neoglycorandomization. Natl. Acad. Sci. USA, 102(35), 12305-12310
• Park, E.H., Kim, Y.J., Yamabe, N., Park, S.H., Kim, H.K., Jang, H.J., Kim, J.H., Cheon, G.J., Ham, J., Kang, K.S. (2014). Stereospecific anticancer effects of ginsenoside Rg3 epi-mers isolated from heat-processed American ginseng on human gastric cancer cell. Ginseng Res, 38(1), 22-27
• [48] Beata S, Barthlomiej M, and Marek R. 2017. The immunomodulatory potential of Leonurus cardiaca extract in relation to endothelial cells and platelets. Innate Immunity, 23(3), 126-139
• David, B. 2019. Lignans. Molecule, 24(7), 1424
• Pereira, R. M., Valadares, P.R., de Oliveira, S.D., de Almeida, V.L., Dias, L. J.C., Shaw, J.M.H., and Gontijo, S. C. 2018. Combined in Vitro Studies and in Silico Target Fishing for the Evaluation of the Biological Activities of Diphylleia cymosa and Podophyllum hexandrum. Molecules, 23, 3303
• Szopa,, Dziurka, M., Warzecha, A., Kubica, P., Klimek-Szczykutowicz, M., Ekiert, H. (2018). Targeted Lignan Profiling and Anti-Inflammatory Properties of Schisandra rubriflora and Schisandra chinensis Extracts. Molecules, 23, 3103
• Rodríguez-García, C., Sánchez-Quesada, C., Toledo, E., Delgado-Rodríguez, M., and Gaforio, J.J. 2019. Naturally Lignan-Rich Foods: A Dietary Tool for Health Promotion? Molecules, 24, 917
• Alexandrine, D., Celine, D., Thomas, R., and Yvan, L. 2012. Among plant lignans, pinoresinol has the strongest antiinflammatory properties in human intestinal Caco-2 cells. J Nutr, 142(10), 1798-805
• Shih-Ming, Q., Yin-Chieh, H., Shu-Ling, F., Lie-Chwen, L., and Chao-Hsiung, L. 2023. Galgravin Isolated from Piper kadsura Ameliorates Lipopolysaccharide (LPS)-Induced Endotoxemia in Mice. Drugs, 9(8), 1841-1848
• Maggioni, D., Garavello, W., Rigolio, R., Pignataro, L., Gaini, R., and Nicolini, G. 2013. Apigenin impairs oral squamous cell carcinoma growth in vitro inducing cell cycle arrestandapoptosis. J. Oncol. 43: 1675-1682
• Woonghee, L., Gwonhwa, S., and Hyocheo, B. 2022. Matairesinol Induces Mitochondrial Dysfunction and Exerts Synergistic Anticancer Effects with 5-Fluorouracil in Pancreatic Cancer Cells. Mar Drugs, 20(8): 473
• Cheeke, P.R., Piacente, S., and Olesze, K.W. 2006. Anti‐inflammatory and anti‐arthritic effects of Yucca schidigera: A review. Journal of Inflammation, 6: 3-6
• Liu, J., Li, Y., Shi, H., Wang, T., Wu, X., Sun, X., and Yu, L. 2016. Components characterization of total tetraploid jiaogulan (Gynostemma pentaphyllum) saponin and its cholesterol‐lowering properties. Journal of Functional Foods, 23:542-555
• Tian, L.W., Zhang, Z., Long, H.L., and Zhang, Y.J. 2017. Steroidal Saponins from the Genus Smilax and Their Biological Activities. Prod. Bioprospect, 7: 283-298
• Frank, T., Netzel, M., and Strass, G. 2003. Bioavailability of anthocyanidin-3-glucosides following consumption of red wine and red grape juice. Can J Physiol Pharmacol, 81(5), 423-435
• Giusti, M., and Wrolstad, R.E. 2013. Acylated anthocyanins from edible sources and their applications in food systems. Biochem Eng J, 14(3), 217-225
• Hock, E.K., Azrina, A., Sou, T., and See, M.L. 2017. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients and the potential health benefits. Food and Nutritional Research, 61(1), 1361779
• Huitrón-Reséndiz, S., Gombart, L., Cravatt, B.F., and Henriksen, S.J. 2001. Effect of oleamide on sleep and its relationship to blood pressure, body temperature and locomotor activity in rats. Experimental Neurology, 172(1): 235-243
• Moghadasian, M. H. 2000. Pharmacological properties of plant sterols in vivo and in vitro observations. Life Sci, 67(6), 605-615
• Saad, B., Nesrine, B., Ilhame, B., Taoufiq, B., Learn-Han, L., Nasreddine, E-O., Ryan, A. S., Khang, W.G., Long, C.M., Abdelhankim, B. (2022).Health Benefits and Pharmacological Properties of Stigmasterol. Antioxidant, 11(10): 1912
• Sherif,, and Hanssan, S. 2020. Brassicasterol with Dual Anti-Infective Properties against HSV-1 and Mycobacterium tuberculosis, and Cardiovascular Protective Effect: Nonclinical In Vitro and In Silico Assessments. Biomedines, 8(5), 132
• Parisa, B., Robab, S., Shaghayegh, K., Masoumeh, K., Behnam, A.L., Javad, H., and Tess, A. 2022. Applications of Essential Oils and Plant Extracts in Different Industries. Molecules, 27(24): 8999
• Jain,L., Patel, S.R., and Desai, M.A. 2022. Patchouli oil: An overview on extraction method, composition and biological activities. J. Essent. Oil Res, 34: 1-11
• Aroiee, H., Babaei, M., Ajdanian, L., Javdani, M., Azizi, M., Asgari, Lajayer, B., and Dell, B. 2022. Effect of Essential Oil of Seven Medicinal Plants on Longevity, Non-germination, and Qualitative and Quantitative Traits of Solanum tuberosum Agria. J. Food Process. Preserv, 46: e16754
• Li-Tiam, M., Yang, B., Peng, C., Kai-Xi, R., Jing, C., Ting, Z., Bo-Yuan, F., Yu, Q., Hong-Yu, Y., Jing-Jie, W., Yu-Quing, L., and Jin, Z. 2022. The analgesic effects of β-elemene in rats with neuropathic pain by inhibition of spinal astrocytic ERK activation. Mol Pain. 18: 17448069221121562
• Varsha, ,  Saya, T., and Rashmi, T. 2023. Hexadecanoic acid methyl ester, a potent hepatoprotective compound in leaves of Pistia stratiotes L. The Applied Biology & Chemistry Journal, 4(4): 118-120
• Lucas, F.S.G., Fernando, P.B., Maria, F.H., Murilo, T.G., Victor, P.R., Patricia, F.D., Jacqueline, C.L. C., Tais, N.C.M., Carlos, A.H.R.K.T., Priscyla, D.M., Jairo, K.B., and Claudia, H.P. 2022. Beta-caryophyllene as an antioxidant, anti-inflammatory and re-epithelialization activities in a rat skin wound excision model. Oxid Med Cell Longev, 2022: 9004014
• De-Sen, L., Lin-Lin, S., Kai, G., Shi-Hong, L., Yan-Chun, L., Yue-Gui, C., Yan, L., and Sheng-Hong, L. 2023. A new sesquiterpene synthase catalyzing the formation of (R)-β-bisabolene from medicinal plant Colquhounia coccinea var. mollis and its anti-adipogenic and antibacterial activities. Phytochemistry, 211: 113681
• Min, H. Y., Mina, L., Amudha, D., Duc, D. L., Chakrabhavi, D. M., Kam, M. H., Gautam, S., and Kwang, S. A. Decanoic Acid Exerts Its Anti-Tumor Effects via Targeting c-Met Signaling Cascades in Hepatocellular Carcinoma Model. Cancers (Basel), 15(19): 4681

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