Many tropical plants have interesting biological activities with potential therapeutic applications. Garcinia mangostana Linn. (GML) belongs to the family of Guttiferae and is named “the queen of fruits”. It is cultivated in the tropical rainforest of some Southeast Asian nations like Indonesia, Malaysia, Vietnam, Philippines, and Thailand. This research aims to determine the concentration of solution mangosteen rind (Garciana mangostana) which is the most effective to healing of black tilapia juvenile (Oreochromis niloticus Bleeker) when infected by A. hydrophila bacteria to produce the highest survival of black tilapia juvenile. This research used experimental method and experimental design by including a complete randomized design (CRD) with five treatments and three replications. The used treatment was soaking black tilapia juvenile for 24 hours in mangosteen rind. The sample infected by A. hydrophila bacteria as much as 0.1 mL/fish at concentration of 108 cfu/mL with intramuscular injection method was used. The concentrations of mangosteen rind solution were 0, 500, 600, 700, and 800 ppm. The parameters observed were clinical symtoms, fish response to feed, fish response to shock, recovery process, and water quality. Data of the clinical symtoms, response fish to feed, response fish to shock, recovery process and water quality were analysed descriptively, while the survival rate data of black tilapia juvenile were analysed using F test at 95% confidence level and continued with Duncan test. The results showed, the use of mangosteen rind solution with 600 ppm concentration was effective to treat tilapia juvenile which infected by A. hydrophila bacteria resulted in the fastest recovery on the third day and the highest survival rate of 91.76.
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