ABSTRACT
The leaf extract of mulberry, Morus alba (L) in ethanol was used to treat the 7,12-
dimethylbenz-anthracene (DMBA) – induced carcinoma in the buccal pouch of Syrian hamster,
Mesocricetus auratus (L). The 0.5 percent DMBA in liquid paraffin was used for painting the buccal
pouch. The DMBA treatment was carried thrice a week for 14 weeks. This was resulted into squamous
cell carcinoma. The parameters analyzed include: The tumor incidence, volume and burden. It was
followed by oral treatment with ethanolic extractives of mulberry leaves (TpEt) at a dosage of 300
mg/kg, body weight, to DMBA (on alternate days for 14 weeks) – painted animals. Administration of
ethanolic extractive of mulberry leaves was found preventing significantly the incidence, volume and
burden of the buccal pouch carcinoma. The ethanolic extractive of mulberry leaves exhibited
significant effect, especially, the antilipidperoxidation and antioxidative enhancement in DMBA
individuals of Syrian hamsters. The results are demonstrating the potency of ethanolic extractive of
mulberry leaves for chemoprevention and significant antilipidperoxidative influence in 7,12-
dimethylbenz-anthracene (DMBA)- induced carcinoma in the buccal pouch of Syrian hamster,
Mesocricetus auratus (L). The activation of caspase through release of cytochrome C through
disruption of mitochondrial membrane potential may be the sequence of action of the active principles
of mulberry leaf extractives. With it’s bioactive compounds, mulberry, Morus alba (L) may open a
new avenue in the cancer prevention and treatment. Taken together, the present attempt provide
experimental evidence that leaves of mulberry may have chemopreventive effects on cancerous
growth. Mulberry, Morus alba (L) may provide a therapeutic option for controlling the growth of
cancer cells.
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