ABSTRACT
Scaffolds are three-dimensional structures used in medicine, especially in tissue engineering, for the reconstruction of damaged tissue or organ. They should be constructed in a special way, to provide support for different cells’ vital functions. Bacterial cellulose produced by Gluconacetobacter xylinus has congruous features to natural occurring extracellular matrixes. Natural pores which occure in cellulose‘s structure have not adequate diameter to colonize them with viable cells. The authors conducted some experiments in order to enlarge channels in cellulose structure. Repeated frosting and defrosting of accurately prepared cellulose samples has created positive results but application of sterile mixture of vegetable oil during culture process gave expected results – diameter of the channels and chambers was big enough to colonize them with viable cells.
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