The aim of the study was to determine the effect of the additive on NAA and BAP on the formation of callus tissue on leaf chamomile explants, cultivar, and to determine the optimal content of said growth regulators for the initiation of callus tissue in vitro. During the experiment fragments of chamomile from the collection of the Department of Genetics, Breeding and Biotechnology of Plant were laid on a medium with mineral composition according to Murashige and Skoog – MS (1962) enriched with auxin NAA in concentrations of 3 and 5 mg·dm-3 and cytokinin BAP in a concentration of 1, 2 , 5, 7 mg·dm-3. Based on the conducted experiment it was found that the initiation of callus chamomile tissue is possible in the case of using leaf explants lined with MS in the combination of auxin NAA in concentration 3 or 5 mg·dm-3 and BAP from 1 to 7 mg·dm-3. The propagation of chamomile callus tissue should be carried out on MS proliferating medium enriched with NAA at a rate of 3 mg·dm-3 and BAP in an amount of 7 mg·dm-3. Explants laid on a medium with this composition develop a large mass of callus tissue.
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