The aim of the work was to decrease the costs of bioconversion process by using chip and simple chromatographic columns containing immobilized C. freundii cells during fermentation. The goal was also modification of medium to control of pH of process in these columns. The purpose of the study was also to ascertain the new systems of biopolymers used for encapsulation of bacterial cells. The stability of obtained membranes in different mediums has been examined. The waste glycerol conversion to 1,3-PD over continuous process has been analyzed as well. The results of the study showed that the double crosslinked alginate beads are better carriers than alginate capsules coated by chitosan during conversion of waste glycerol to 1,3-PD. Unfortunatrly these two kinds of capsules should not be used for continuous process and repeated bioconversions because of low mechanical properties of the beads. PDADMAC beads and modified (e.g. by additions) PDADMAC capsules should not be used for continuous and repeated bioconversions as well.
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