An efficient in vitro regeneration and conservation system depend on the healthy culture induction from the suitable explants. Culture induction is a significant stage when very small plant material exists from the rare species. It is necessary to develop culture induction protocol from various explants to conserve the valuable plant species. Atalantia monophylla is a rare species with life giving properties. Shoots were induced from the shoot tip, node and internode explants. Among these the nodal shoot segments were reported most appropriate explant for the induction of shoots from the nodal meristems. MS medium (Murashige and Skoog) proved better than the Woody Plants (WP) medium in bud breaking. Sucrose at 3% level was optimum concentration for the establishment of cultures than the other concentrations evaluated. Shoot tips responded on MS medium supplemented with 0.5 mg L-1 each of BAP and Kin, nodal shoot segments responded better on MS medium augmented with 1.0 mg L-1 BAP . Half strength MS medium supplemented with 2,4-D (1.0 mg L-1) induced maximum responsive callus (87%) from the internode explants.
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