The phytoconstitution, anti-oxidant and the iron chelating profiling of 30% acetone extract of crude honey residue collected from Obudu Local Government Area of Cross Rivers State, Nigeria have been investigated. The crude extract was obtained by cold extraction using 30% acetone in ethyl acetate at room temperature, freeze dried and stored below room temperature. The crude extract phytoconstitution profiling was done using high performance liquid chromatography, HPLC and qualitative screening technique. Its anti-oxidant and iron chelating profiling were probed using standard procedures. The HPLC chromatogram reveals the presence of ferulic acid, rutin, apigenin, quercetin and kaempferol at 20.954 min, 23.312 min, 30.194 min, 36.698 min and 39.223 min retention time respectively. The phytochemicals present include terpenoids, flavonoids, glycosides and phenols. The anti-oxidant activity of the crude extract increased as its concentration increased. The iron chelating activity witnessed an increase as its concentration increased. The chelating activity of the crude extract was relatively lower when compared to those of the EDTA standard. The study has established the phytoconstitution, anti-oxidant and iron chelating profiling of the 30% acetone extract of crude honeybee residue.
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