ABSTRACT
Sessile organisms like plants have drastically reduce possibility to avoid harmful influence of the natural habitat. Evolution of first land plants allowed adapting to harsh conditions, yet despite broad range of tolerance factors such as excessive light and temperature impose limitation for biological activity because viability and in the case of crops the productivity is limited. In fact, physiologists make lots of efforts to increase stress tolerance searching and selecting new cultivars of known crops. As in most cases the exact mechanism of reached improvement is still missing selection is occupied by immense amount of time, energy and funds. Proposed in paper technique of stress resistance estimation is inexpensive and highly reproducible solution for crop selection even at early stage of development. It is based on well-known fluorescent assessment (PAM) of plant photosynthetic machinery expose to factor of interest. We tested two barley (Hordeum vulgare L.) cultivars spring Carina and winter Lomerit exposed to thermal stress and estimated maximal (Fv/Fm) and effective photosystem II quantum yield precisely specifying the way absorbed energy is utilized by photochemical (ΦPSII) or regulated (ΦNPQ) and non-regulated (ΦNO) non-photochemical manner. Studies have confirmed various heat-resistance of tested barley cultivars and proved PAM‑technique utility.
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