In vitro propagation through ex vitro rooting of a medicinal spice Piper longum Linn.

ABSTRACT
Successful in vitro propagation through ex vitro rooting mechanism has been achieved in Piper
longum by nodal shoot segment cultures. Shoot tip explants were less proliferative compared to the
nodal meristems. The explants were sterilized using 0.1% HgCl2 and cultured on Murashige and Skoog
(MS) medium with various concentrations of growth regulators. MS medium supplemented with 1.0
mg/L 6-benzylaminopurine (BAP) was found suitable for bud breaking within four weeks. Kinetin
(Kin) was not reported impressive compared to BAP in culture induction response. The shoots were
multiplied and elongated on MS medium supplemented with 0.5 mg/L each of BAP and Kin and 0.1
mg/L indole-3 acetic acid (IAA), where the shoots were elongated up to 5.8 cm length. Rooting and
acclimatization was achieved by ex vitro rooting methods using 300 mg/L indole-3 butyric acid (IBA)
for 5 min, and 5.3 roots with 3.2 cm average length were observed. The rooted shoots were transferred
to the greenhouse for acclimatization. The hardened plantlets were transferred to earthen pots and
maintained in the greenhouse. Normal flowering was observed in micropropagated plants of P.
longum

References
[1] T. K. Biswas, L. N. Maity, B. Mukherjee. International Journal of Low Extreme
Wounds 3 (2004) 143-150.
[2] K. K. N. Nair. Manual of non-wood forest produce plants of Kerala, Kerala forest
research institute, Kerala, 2000.
[3] A. B. Chaudhuri. Daya Publishing House, 2007.
[4] J. L. N. Shastry, Ayurvedokta Aushadha Niruktamala, Chaukhambha orientaliya,
Varanasi; 2001, p. 69.
[5] Williamsons. Elsevier Health Science (2002) 225-227.
[6] M. Ashalatha, B. S. Rekha. International Ayurvedic Medicinal Journal 3 (2015) 2841-2849.
[7] Anonymous. New Delhi 4 (2004) 91-93.
[8] T. V. Viswanathan. Long pepper in Chadha, K.L. and Gupta R. (Ed.). 1995.
[9] A. Chatterjee, C. Dutta. Science and Culture 29 (1963) 568.
[10] A. Dasgupta, P. C. Dutta. Quarterly Journal of Crude Drug Research 18 (1980) 17-20.
[11] P. Madhusudan, K. L. Vandana. Biochemistry and Systematic Ecology 29 (2001) 537-539.
[12] S. E. Lee, B. S. Park, K. M. Kim. Crop Protection 20 (2001) 523-525.
[13] C. Khushbu, S. Roshni, P. Anar, M. Carol, P. Mayuree. International Journal of
Research in Ayurveda and Pharmacy 2 (2011) 157-161.
[14] K. Joshi, K. Panara, K. Nishteswar, S. Chaudhary. International Journal of
Pharmaceutical Sciences 4 (2013) 3617-3627.
[15] V. Arya. Orient Lonhman, 1995.
[16] M. S. Heera, T. S. Mahesh, B. Thushar, S. R. Rao. International Research Journal of
Pharmacy 5 (2014) 713-716.
[17] A. Sharma, R. Singh. Bulletin Medicinal and Ethnobotanical Research 2 (1980) 262.
[18] A. Bhargava, C. Chauhan. Indian Journal of Pharmacy 30 (1968) 150.
[19] N. Sawangjaroen, K. Sawangjaroen, P. Poonpanang. Journal of Ethnopharmacology 91
(2004) 357-360.
[20] V. K. Kulshresta, N. Singh, R. K. Shrivastava, R. P. Kohli, S. K. Rastogi. Journal of
Research in Indian Medicine 6 (1971) 17-19.
[21] P. Devan, S. Bani, K. A. Suri, N. K. Satti, G. N. Qazi. International Journal of
Immunopharmacology 7 (2007) 889-899.
[22] A. H. Shah, A. H. Al-Shareef, A. M. Ageel, S. Qureshi. Plant Foods for Human
Nutrition 52 (1998) 231-239.
[23] A. K. Agarwal, M. Singh, N. Gupta. Journal of Ethnopharmacology 44 (1994) 143-
146.
[24] K. S. Natarajan, M. Narasimhan, K. R. Shanmugasundaram, E. R. Shanmugasundaram.
Journal of Ethnopharmacology 105 (2006)76-83.
[25] G. Vedhanayaki, G. V. Shastri, A. Kuruvilla. Indian Journal of Experimental Biology
41 (2003) 649- 651.
[26] C. R. Pradee, G. Kuttan. Journal of Clinical and Experimental Metastasis 19 (2002)
703-708.
[27] K. Selvendiran, D. Sakthisekaran D. Biomedical and Pharmacotheraphy 58 (2004) 264-
267.
[28] A. L. Seon, S. H. Seong, H. H. Xiang, S. H. Ji, J. O. Gab, S. L. Kyong, K. L. Myung, Y.
H. Bang, S. R. Jai. Chemical and Pharmaceutical Bulletin 53 (2005) 832-835.
[29] P. N. Krishnan, S. W. Decruse, R. K. Radha. In Vitro Cell and Developmental Biology
of Plant 47 (2011) 110-122.
[30] Demand and supply of medicinal plants in India. NMPB-FRLHT, Published by
Bishensingh Mahendrapal singh, 2008.
[31] K. K. Ranaweeraa, M. T. K. Gunasekarab, J. P. Eeswara. Scientia Horticulturae 155
(2013) 8-14.
[32] A. K. Patel, M. Phulwaria, M. K. Rai, A. K. Gupta, S. Smita, N. S. Shekhawat. Scientia
Horticulturae 165 (2014) 175-180.
[33] H. Yan, C. Liang, L. Yang, Y. Li. Acta Physiologiae Plantarum 32 (2010) 115-120.
[34] P. Baskaran, J. Van Staden. South African Journal of Botany 86 (2013) 46-50.
[35] K. Ram. N. S. Shekhawat. Physiology and Molecular Biology of Plants 17 (2011) 281-
289.
[36] P. Meena, N. S. Shekhawat. Phytomorphology 62 (2012) 1633-169.
[37] M. Phulwaria, N. S. Shekhawat. Physiology and Molecular Biology of Plants 19 (2013)
435-441.
[38] J. P. R. Martins, E. R. Schimildt, R. S. Alexandre, B. R. Santos, G. C. Magevski.
Pesquisa Agropecuaria Tropical 43 (2013) 138-146.
[39] M. S. Shekhawat, N. Kannan, M. Manokari, C. P. Ravindran. Journal of Genetic
Engineering and Biotechnology 13 (2015c) 209-214.
[40] M. S. Shekhawat, M. Manokari. Chinese Journal of Biology. 2015
doi.org/10.1155/2015/273405.
[41] D. Lodha, A. K. Patel, N. S. Shekhawat. Physiology and Molecular Biology of Plants,
2015. DOI 10.1007/s12298-015-0310-6.
[42] M. S. Rathore, S. Yadav, P. Yadav, J. Kheni, B. Jha. Biomass and Bioenergy 83 (2015)
501-510.
[43] E. V. Soniya, M. R. Das. Plant Cell, Tissue and Organ Culture 70 (2002) 325-327.
[44] T. Murashige, F. Skoog, Physiologia Plantarum 15 (1962) 473–497.
[45] M. Sharon, G. Maurya. United States Patent. US 7,402,433 B2, Jul.22, 2008.
[46] M. Kehie, S. Kumaria, P. Tandon. Biotechnology 2 (2012) 31-35.
[47] N. Fatima, M. Anis. Physiology and Molecular Biology of Plants 18 (2012) 59-67.
[48] M. Phulwaria, A. K. Patel, J. S. Rathore, K. Ram, N. S. Shekhawat. Acta Physiologia
Plantarum. 2014. DOI 10.1007/s 11738-014-1486-z.
[49] S. Ghatge, S. Kudale, G. Dixit. Asian Journal of Biotechnology 3 (2011) 397-405.
[50] M. S. Shekhawat, M. Manokari. Advances in Biology, 2015, DOI:
10.1155/2015/639678.
[51] M. S. Shekhawat, N. Kannan, M. Manokari, M. P. Ramanujam. Journal of Sustainable
Forestry 33 (2014) 327-336.
[52] I. O. Ezeibekwe, C. L. Ezenwaka, F. N. Mbagwu, C. I. N. Unamba. New York Science
Journal 2 (2009) 1-8.
[53] M. S. Rathore, N. S. Shekhawat. Agricultural Science Research Journal 1 (2011) 191-
198.
[54] M. S. Shekhawat, M. Manokari, N. Kannan, A. Pragasam. The Pharma Innovation 1
(2012) 1-7.
[55] M. Phulwaria, M. K. Rai, A. K. Patel, V. Kataria, N. S. Shekhawat. AoB Plants, 2013,
doi:10.1093/aobpla/pls054.
[56] A. K. Patel, T. Agarwal, M. Phulwaria, V. Kataria, N. S. Shekhawat. Industrial Crops
and Products 52 (2014) 499-505.
[57] M. S. Shekhawat, N. Kannan, M. Manokari, Industrial Crops and Products 100 (2015a)
43-50.
[58] M. S. Shekhawat, N. Kannan, M. Manokari, C.P. Ravindran. Journal of Applied
Research on Medicinal and Aromatic Plants 2 (2015b) 174-181.
[59] A. Aygun, H. Dumanoglu. Frontier in Plant Science 6 (2015) 225.

Download all article in PDF

WSN 37 (2016) 12-24