The aim of the study was to determine an optimal composition of growth media for Drosera rotundifolia L. cultured in vitro. Adventitious shoots of Drosera rotundifolia originating from an established in vitro culture were placed on complete Murashige and Skoog (MS) (1962) medium, MS medium with nutrient content reduced to ¾ MS, ½ MS, ¼ MS, 1/6 MS and MS medium with selected macronutrient (KNO3, KNO3 + NH4NO3, KH2PO4 or CaCl2) content reduced by ½. The medium containing ¼ of standard MS nutrients was found the most effective, as plants in this variant had the greatest weight and developed the greatest number of adventitious shoots (37.1). It was also conducive to root growth and development. Flowering was only observed on complete MS, ¾ MS and ½ MS media and MS with reduced content of KNO3 or KNO3 + NH4NO3. Reducing mineral content to ¼ MS or more resulted in red coloration of glandular tentacles.
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