Berseem clover (Trifolium alexandrinum L.) is one of the most important forage crops in Iran. In order to determine yield, yield components and qualitative characteristics of two cultivars of berseem clover in low input nitrogen farming in 2015 and 2016, two factor randomized complete block design with split plot combined analysis over time and four replications was used in Isfahan, Iran. The cultivars were Sacronomt and Multicut, and the nitrogen application levels were 10, 40 and 70 kg ha-1. Herein, higher values of plant height, total fresh yield, total dry yield, nitrate, protein, organic matter and ash were seen in 2015, yet, higher phosphorous, potassium, Fe, Mn, Zn and Cu were evident in 2016. Moreover, Sacromont cultivar generated higher values of total fresh yield, total dry yield and Fe, while higher values of plant height, nitrate, protein, phosphorous, potassium, organic matter, ash, Mn, Zn and Cu were noted in Multicut. Increasing the nitrogen fertilizer application rate from 10 to 70 kg N ha-1 resulted in numerically increased plant height, total fresh and dry yield. Maximum nitrate, protein, potassium, organic matter, ash and Zn levels were obtained in an application of 70 kg N ha-1. Over all, nitrate concentration in the crop increased step by step with the addition of nitrogen fertilizer. However, the highest values of phosphorous, Mn and Cu were achieved in an application of 40 kg N ha-1. Nitrogen fertilizer management is, thus, one of the most important inputs in achieving high productivity and yield of berseem clover. The appropriate management of nitrogen is, therefore, important in cropping systems and a balance between applied levels is essential for normal plant growth and human consumption. All in all, both forage crop production and quality is vital for successful animal production in sustainable agriculture systems. On the basis of the results of this study, it could be concluded that application of 70 kg N ha-1 is good for both cultivars.
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