The present study states about the biosynthesis and UV-Visible characterization of Zinc oxide (ZnO) nanoparticles using leaf, stem, roots and inflorescence of the medicinal herb Euphorbia hirta. The aqueous extracts of plant parts were acted as capping and reducing agent while Zinc Nitrate Hexahydrate used as precursor. One milliliter of plant extracts mediated the formation of ZnO nanoparticles in solution which were visually confirmed by the development of yellowish color in the reaction mixtures. The optical properties of the reaction mixtures were analyzed by the UV-Visible double beam spectrophotometer. The leaf reaction mixture showed strong absorption band at 302nm, stem reaction mixture at 299nm, and the root and inflorescence reaction mixtures at 310nm. The results indicate that whole Euphorbia hirta plant can be used for the biosynthesis of Zinc oxide nanoparticles.
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