ABSTRACT
The study involved synthesis of Zinc Oxide nanoparticles using biological and chemical
reducing agents. The aim was to compare the yield, nature and antimicrobial activity of nanoparticles.
Nanoparticles synthesized by the two methods were characterized by FTIR Spectroscopy, Scanning
Electron Microscopy (SEM), Energy Dispersive Xray (EDAX), Transmission Electron Microscope
(TEM) and X-ray diffraction (XRD). In biological method, Pithecellobium dulce and Lagenaria
siceraria leaf extract; and in chemical method, sodium hydroxide was used as reducing agents.
Antibacterial study was carried out on gram-positive and gram negative bacterial strains by disc
diffusion method. The biologically synthesized ZnO Nanoparticles showed better antimicrobial
activities with respect to the activities of synthetic drugs than chemical method. Antioxidant potential
of synthesized nanoparticles was assessed through 2,2–diphenyl-1-picrylhydrazyl (DPPH) method.
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WSN 18 (2015) 49-59WSN 18 (2015) 60-77
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