ABSTRACT
This study investigated the biocatalytic bioremediation of polycyclic aromatic hydrocarbons in crude oil contaminated soil by garbage derived from fermented organic peel wastes. The fruit garbage used were ripened plantain, banana, pineapple, and watermelon peel wastes, which were fermented for 90 days for the derivation of enzymes. Six soil samples were collected and labeled; group 1-6. Group served as positive control, group 2 served as untreated soil sample, while group 3-6 treated with 30, 50, 70, and 100% of fermented garbage enzyme solution for 30, 60, 90, 120, 150, and 180 days. PAHs were determined based on standard methods. GE at 30% from 120 to 180 days elicited mean removal of naphthalene at 65.03±0.03, 78.02±0.02, and 85.62±0.02 % respectively and similar results were observed in treatment with 70 and 100% garbage enzyme solution. Treatment with 30% of garbage for 120 to 180 days elicited mean removal of anthracene at 57.03±0.03, 62.93 ±0.03, and 82.14±0.02 % respectively. GE at 30% from 120 to 180 days elicited mean removal of fluoranthene at 60.82±0.03, 68.26 ±0.02, and 74.52 ±0.03 % respectively. Similar results were obtained on mean percentage removal of pyrene, 1-2-benzanthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benz[a]pyrene, indeno (1-2-3-cd) pyrene, and benzo(g-h-i)perylene after treatment with 30, 70, and 100 % of garbage enzyme ferments. The differences observed in the biocatalytic bioremediation activities of the applied fruit garbage enzymes might be due to the difference in composition of the garbage enzyme mixtures arising from the degree of microbial activities in the solutions.
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