In this paper, the effect of pH on nanoparticles (AgNPs) using plants extracting has been investigated. The aqueous sol of AgNPs prepared at different pH values using hydrothermal method display different Surface Plasmon Resonance (SPR) behavior and the maximum absorption values were at pH = 14. AgNPs were characterized using X-Ray diffraction, UV-Vis spectroscopy and scanning electron microscope. Face-center cubic Ag nanoparticles with crystal size about 3.86 nm have been noticed. The absorption band showed that Ag has sharp curves in the ultraviolet and at the edge of the visible region. The SEM images showed cluster shaped nanoparticles, and when increasing the pH values, the result revealed the formation of larger nanoparticles cluster with more accurate crystallite sizes. The antimicrobial and antifungal activity was performed by Agar well diffusion assay against Escherichia coli, Bacillus subtilis and Candida albicans. The diameter of the inhibition zones of Ag NPs against the bacterial strains such as, Bacillus subtilis (31 mm) and Escherichia coli (30 mm) at 200 µg/ml concentration and the diameter of the inhibition zones of Ag NPs against the fungus strains such as, Candida albicans (36 mm) at the same concentration.
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