Application of High-Energy Laser coupled with Gamma – Lonizing Irradiations as New Sterilizing Technique

ABSTRACT
The present investigations introduce for first time new trend of coupling of two sterilizing
sources, high energy Nd-Laser and Gamma – irradiations which has oxidative nature by additional to
its thermal effects. The investigated samples will be examined before and after radiations to confirm
its internal structure (XRD, microstructure (SEM, AFM, Raman-Spectra) and killing ratio for polluted
bacteria. Furthermore some selected biological tests will be checked to be sure from toxicity ratio after
radiations. Also many of investigational parameters will be tested such as strength of irradiation dose
and irradiation dosage time to achieve maximum healthy sterilizing ratio per minimum time to validate
application and wide scale application of this promising sterilizing technique.

References
[1] Ká tia Aparecida da Silva Aquino. Sterilization by Gamma Irradiation, Gamma
Radiation, Prof. Feriz Adrovic (Ed.), ISBN: 978-953-51-0316-5, InTech, Available
from: http://www.intechopen.com/books/gammaradiation/sterilization-by-gammairradiation. (2012).
[2] Action biologique et chimique des rayonnements ionisants (ABCRI). B. Tilquin (Ed.),
115 pp. Paris, France (2001).
[3] Al-Kahtani, H. A. Abu-Tarboush, M. H. Bajaber, A. S. Atia, M. Abou-Arab, A. A.& ElMojaddidi, M. A. Chemical changes after irradiation and post-irradiation, storage in
Tilapia and Spanish mackerel. Journal of Food Science, 61 (1996) 729-733.
[4] Alariqi, S.A.S. Pratheep, A. K. Rao, B. S. M. & Singh, R. P., Biodegradation of
Gamma-sterilised biomedical polyolefins under composting and fungal culture
environments. Polymer Degradation Stability, 91, 6, (2006) pp. 1105–1116.
[5] American Society for Testing and Materials. Standard Guide for Selection and
Calibration of Dosimetry Systems for Radiation Processing, ISO/ASTM 51261,Annual
Book of ASTM Standards, ASTM, p.p 970–988, USA, New York, (2006).
[6] Freitag, D. Grigo, U. Mueller, P. Polycarbonates. In: Encyclopedia of Polymer Science
and Engineering, Vol. 11, 2nd ed, 648-718, New York, Wiley (1988).
[7] Aquino, K. A. S. & Araujo, E. S. ,Effects of a Hindered Amine Stabilizer (HAS) on
Radiolytic and Thermal Stability of Poly(methyl methacrylate). Journal of Applied
Polymer Science, 110, 1, (2008). pp. 401-401.
[8] Aquino, K. A. S. Araujo, E. S & Guedes, S. M. Influence of a Hindered Amine
Stabilizer on optical and mechanical properties of poly (methyl methacrylate) exposed
to gamma irradiation. Journal of Applied Polymer Science, 116, 2, (2010). pp.748-753.
[9] Araujo, E. S. Khoury, H. J. & Silveira, S. V. Effects of gamma-irradiation on some
properties ofdurolon polycarbonate. Radiation Physics and Chemistry, 53 (1998)79-84.
[10] Armstrong, S. G. Wyllie, S. G. & Leach, D. N. Effects of preservation by gamma
irradiation on the nutritional quality of Australian fish. Food Chemistry, 50 (1994) pp.351-357.
[11] Baccaro, S. Brunella, V. Cecيlia, A. & Costa, L. ,Gamma- irradiation of poly(vinyl
choride) for medical applications. Nuclear Instruments and Methods in Physics
Research, 208 (2003) pp. 195-198.
[12] [12] Brewer, M. S. Irradiation effects on meat flavor: A review. Meat Science, 81, 1,(2009). pp. 1-14.
[13] [13] Billen, D. The role of hydroxyl radical scavengers in preventing DNA strand
breaks induced by X irradiation of toluene treated E. coli., Radiation Research, 97, (1984) 626-629.
[14] Borrely, S. I. Cruz, A. C. Del Mastro, N. L. Sampa, M. H. O. & Somessari, E. S.
Radiation processing of sewage and sudge. A review. Progress in Nuclear Energy, 33,
(1998) pp. 3-21.
[15] Broomfield, S. Hryciw, T. & Xiao, W.. DNA postreplication repair and mutagenesis in
Saccharomyces cerevisiae. Mutation Research, 486, (2001) pp. 167-184.
[16] [16] Cadet, J. Douki, T. Gasparutto, D. Gromova, M , Pouger, J. P. Ravanat, J. L.
Romieu, A. &Sauvaigo S. Radiation-induced damage to DNA: mechanistic aspects and
measurement of base lesions. Nuclear Instruments and Methods. in Physics Research
section B, 151, (1999) pp. 1-7.
[17] Campbell, D.G. & Li P. Sterilization of HIV with irradiation: relevance to HIV infected
bone allografts. NZ Journal of Surgery, 69, (1999) pp. 517-521.
[18] Coates, M. E. Ford, J. E. Gregory, M. E & Thompson, S.Y. Effects of gammairradiation on the vitamin content of diets for laboratory animals. Laboratory (1969).
[19] Crucq A. S. Slegers C. Deridder V. & Tilquin B., Radiosensitivity study of cefazolin
sodium. Talanta, 52, (2000) 873-877.
[20] Daar, E. S. Moudgil, Meyer, R. D.& Ho, D. D., Transient levels of viremia in patients in
primary human immunodeficiency virus type 1 infection. New England Journal of
Medicine. 324, (1991) 961-964.
[21] Daly M. J. Gaidamakova, E. K. Matrosova, V.Y. Vasilenko, A. Zhai, M. Leapman, R.
D. Lai, B. Ravel, B. S.W., Kemner, K. M. & Fredrickson J.K. Protein oxidation
implicated as the primary determinant of bacterial radioresistance. PLOS Biology, 5, (2007) 769-779.
[22] Diehl, J.H., Combined effects of irradiation, storage, and cooking on the vitamin Eand
B1 levels of foods. Food Irradiation, 10 (1967) 2-7.
[23] Farkas, J. & Farkas C. M. (2011). History and future of food irradiation, Trends Food
Science &Technology, 22, 2-3, pp. 121-126.
[24] Fox, J. B. J. Lakritz, L. & Thayer, D. W. Thiamin, riboflavin and γ -tocopherol retention
in processed and stored irradiated pork. Journal of Food Science, 62, 5, (1997) 1022-1052
.[25] Fox, J. B. J. Lakritz, L. Hampson, J. Richardson, F. Ward, K & Thayer, D. W.Gamma
irradiation effects on thiamin and riboflavin in beef, lamb, pork, and turkey. Journal of
Food Science, 60, 3, (1995) 596-598.
[26] Geis, P. A. & Szaniszlo, P. J.. Carotenoid pigments of the dermatiaceous fungus
Wangiella dermatitidis. Mycologia, 76, (1984) 268-273.
[27] Goldman, M. Gronsky, R. & Pruitt, L. The influence of sterilization technique and
ageing on the structure and morphology of medical-grade ultrahigh molecular weight
polyethylene. Journal of Materials in Medicine, 9, (1998) 207- 212.
[28] Gonzalez, M E. & Docters, A. S. Evaluation of stability of polymeric insulation
materials in radiation fields and development of radiation stable PVC and
polypropylene for medical devices, In: Stability and stabilization of polymers under
irradiation, IAEA tecdoc- 1062 (Ed), 97-110, Vienna, Austria (1999).
[29] Guillet, J. Polymer photophysics and photochemistry, Cambridge University Press, New
York, USA,(1985).
[30] Grecz, N. Snyder, O. P. Walker A. A. & Anellis A. Effect of temperature of liquid
nitrogen on radiation resistance of spores of Clostridium botulinum. Applied
Microbiology, 13, (1965) 527–536.
[31] Greez, N. Rowley, D.B. & MATSUYAMA, A. The action of radiation on bacteria and
viruses, In: Preservation of Food by Ionizing Radiation, Vol. 2, Josepson, E. S.
&Peterson, M. S. (Eds), 167, CRS Press, Boca Raton FL, USA Grieb, T. A. Forng, R.
Y. Stafford, R. E., Lin, J. Almeida, J. Bogdansky, S. Ronholdt, C. Drohan, W. N. &
Burgess, W. H. (2005). Effective use of optimized, high dose (50kGy) gamma
irradiation for pathogen inactivation of human bone allographs. Biomaterials, 26,
(1983) 2033-2042.
[32] Hafez, Y. S. Mohamed, A. I. Singh, G. & Hewedy, F. M. Effects of gamma irradiaton
on proteins and fatty acids of soybean. Journal of food science, Vol. 50, (1985). pp.
1271- 1274; Haghparast, S. Kashiri, H. Shabanpour, B. & Pahlavani, M. H.
Antioxidantproperties of sodium acetate, sodium citrate and sodium lactate on lipid
oxidationin rainbow trout (Oncorhynchus mykiss) sticks during refrigerated storage
(4°C). Iranian Journal of Fisheries Sciences, 9, (2010). pp. 73-86.
[33] Hansen M.T. Multiplicity of genome equivalents in the radiation-resistant bacterium
Micrococcus radiodurans. J. of Bacteriology, 134, (1978) pp. 71-75.
[34] Hau, L. B. & Liew, M. S. Effects of gamma irradiation and cooking on vitamin B6 and
B12 in grass prawns (Penaeus monodon). Radiation Physics and Chemistry, 42, (1993) 297-300.
[35] Helfinstine S. L. Vargas-Aburto C. Uribe R.M. & Woolverton C. J. Inactivation of
Bacillus endospores in envelopes by electron beam irradiation. Applied and
Environmental Microbiology, 71, (2005) 7029-7032.
[36] Hernigou, P. Gras, G. Marinello, G. & Dormont, D. Influence of irradiation on the risk
of transmission of HIV in bone grafts obtained from appropriately screened donors and
followed by radiation sterilization, Cell Tissue Bank, 1 4, (2000) 279-289.
[37] Hiemstra, H. Tersmette, M. Vos, A. H. V. Over, J. Van Berkel, M. P. & Bree, H.
Inactivation of HIV by gamma radiation and its effect on plasma and
coagulationfactors, Transfusion, 31, 1, (1991) 32-39.
[38] International Atomic Energy Agency (IAEA). International standards for tissue banks,
AEA, Vienna, Austria (2004).
[39] International Atomic Energy Agency (IAEA). Radiation for a clean environment,
Proceedings of International Symposium on the use of high-level radiation in
wastetreatment, Munich, Germany, March 17-21, (1975).
[40] International Organization for Standardization. Sterilization of healthcare products –
Requirements for validation and routine control – Radiation sterilization, ISO 11137,
ISO, Geneva, Switzerland, (2006).
[41] International Organization for Standardization. Sterilization of Health Care Products —
Radiation — Part 1: Requirements for the Development, Validation and Routine
Control of a Sterilization Process for Medical Products, ISO 11137-1, ISO, Geneva,
Switzerland, (2006).
[42] International Organization for Standardization. Sterilization of Health Care Products —
Radiation — Part 2: Establishing the Sterilization Dose, ISO 11137-2, ISO, Geneva,
Switzerland, (2006).
[43] International Organization for Standardization. Sterilization of Health Care Products —
Radiation — Part 3: Guidance on Dosimetric Aspects, ISO 11137-3, ISO, Geneva,
Switzerland(2006).
[44] International Organization for Standardization. Sterilization of Medical Devices –
General Requirements for Characterization of a Sterilizing Agent and the Development,
Validation and Routine Control of a Sterilization Process for Medical Devices, ISO14937(E), ISO, Geneva, Switzerland,(2000).
[45] Kaplan, I.. Nuclear Physics. Addison-Wesley Pub. Co., Boston, USA Katta, S. R. Rao,
D. R. Sunki, G. R. & Chawan, C. B. (1991). Effects of gamma irradiation of whole
chicken carcasses on bacterial loads and fatty acids. Journal of food science, 56, (1955)
pp. 371-372.

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