In vitro antioxidant activity of green-synthesized silver nanoparticles (AgNPs), silver-zinc oxide nanoparticles (Ag-ZnO NPs) using leaf extracts of Corallocarpus epigaeus

https://doi.org/10.65770/IWFL9183

ABSTRACT

The present study evaluates the in vitro antioxidant capacity of silver nanoparticles (AgNPs), green silver–zinc oxide nanoparticles (Ag–ZnO NPs), and the methanolic leaf extract of Corallocarpus epigaeus. The in vitro antioxidant activity was determined by the H₂O₂ radical-scavenging assay, reducing power assay, and DPPH radical-scavenging assay. The methanolic leaf extract and standard ascorbic acid were prepared accordingly and used to assess antioxidant potential using the H₂O₂ radical-scavenging assay, reducing power assay, and DPPH radical-scavenging assay. UV-Vis spectrophotometry confirmed the biosynthesis of silver and silver–zinc oxide NPs. UV-Vis spectrophotometry confirmed the green-synthesized AgNPs and Ag-ZnO NPs by surface plasmon resonance at 460 nm and 450 nm, respectively. The fabricated Ag-ZnO NPs showed significant antioxidant activity compared with AgNPs and the leaf extract in the H₂O₂ radical-scavenging assay (72.33±0.21%), reducing power assay (0.325±0.12), and DPPH radical-scavenging assay (79.49±0.15%), in a dose-dependent manner. Therefore, in the present investigation, the antioxidant potential of Ag-ZnO NPs can be used as an alternative antioxidant, and antioxidants can mitigate the harmful effects of reactive oxygen species (ROS).

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WSN 216 (2026) 108-120

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