ABSTRACT
Antimicrobial properties and phytochemical analysis of Psidium gujava leaf extract against Pseudomonas aeruginosa was carried out. Fourier transform infrared and Gas chromatographic analysis showed that the extracts contain the following phytochemicals which include proanthocyanin, lunamarin, quinine, epihedrine, anthocyanin, flavan, sapogenin, phenol, flavonones, naringenin, steroids, epicatechin, kaempferol, phytate, flavone, oxalate, catechin, resveratrol and tannin. Among the plant extracts tested, ethanol and hot water extract of Psidium gujava showed the highest inhibition on Pseudomonas aeruginosa For twitching motility, hot water and ethanolic extracts of exerted the highest inhibition, Hot water extract showed the best inhibition in biofilm formation and cell adhesion, In protelytic activity, cold water extract showed the highest inhibition for psidium gujava, on Pseudomonas aeruginosa while ethanol had the best inhibition in pyocynin production. The present study revealed the potential for psidium gujava leave extracts in treating microbial infections through inhibition of quorum sensing regulated virulence factors for the prevention of bacterial infections.
References
- Davey M.E. and O’Toole G.A. (2000). Microbial biofilms: from ecology to molecular genetics. Microbiol .Mol. Biol. Rev. 64, 847-867.
- Donlan R.M. (2002). Biofilms: Microbial life on surfaces. Emerging Infect. Dis. 8, 881-890.
- Fuqua C., Parsek M.R. and Greenberg E.P. (2001). Regulation of geneexpression by cell-to-cell communication: acyl-homoserinelactone quorum sensing. Annu Rev Genet, 35: 439-468.
- Fux C (2005). Survival strategies of infectious biofilms. Trends Microbiol. 13: 34-40
- George E.A. and Muir T.W. (2007). Molecular Mechanisms of agr Quorum Sensing in Virulent Staphylococcus aureusylococci. Chem-BioChem. 8(8): 847-55. doi: 10.1002/cbic.200700023
- Heukelekian H.and Heller A. (1940). Relation between food concentration and surface for bacterial growth. Bacteriol. 40, 547-558.
- Hibbing M.E. and Fuqua C. (2012). Inhibition and dispersal of Agrobacterium tumefaciens biofilms by a small diffusible Pseudomonas aeruginosaaeruginosaexoproduct(s). Arch Microbiol; 194(6): 391-403.
- Karthick R.N.S. and Vivek J.M. (2016). Anti-quorum sensing activities of medicinal plant extracts against quorumsensing mediated virulence factors of Pseudomonas aeruginosaaeruginosa. Der Pharmacia Lettre, 8 (8): 412-423
- Khan S. (2014). Isolation of Shigella species and their resistance patterns to a panel of fifteen antibiotics in mid and far western region of Nepal. Asian Pacific J Tropical Dis. 4: 30-34.
- Kumar J.S., Umesha S., Prasad K.S., Niranjana P. (2016). Detection of quorumsensing molecules and biofilm formation in Ralstoniasolanacearum. Current Microbiol, 72(3): 297-305.
- Muhsin J., Ufaq T., Tahir H. and Saadia A. (2015). Bacterial Biofilm: Its Composition, Formation and Role in Human Infections. Research & Reviews: Journal of Microbiology and Biotechnology, 18, 87–92.
- Rasko D.A., and Sperandio V. (2010). Anti-virulence strategies to combat bacteria-mediated disease. Nature reviews Drug discovery, 9(2): 117-28. doi: 10.1038/nrd3013
- Rutherford S. T.and Bassler B.L. (2012). Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring HarbPerspect Med. a012427.
- Schauder S. and Bassler B.L. (2003). The languages of bacteria. Genes Dev 15: 1468-80.
- Srey S., Jahid I.K. and Ha S.D. (2013). Biofilm formation in foodindustries: a food safety concern. Food Control 31, 572–585
- Zobell, C.E. (1943). The effect of solid surfaces upon bacterial activity. Bacteriol. 46, 39-56
Download all article in PDF
