ABSTRACT
Phytochemical screening and assessment of antibacterial and antifungal effects of Indigofera suffruticosa (locally known as Uri aturu). Whole fresh plants of Indigofera suffruticosa were collected from a non- immigrated cultivable land area along futo road, Imo state, Nigeria. It was authenticated and sent to the laboratory for analysis. The plant leaves were processed by washing, drying at room temperature and mechanically grinded into powder. Ethanol methanol and aqueous extracts of the crude powder were respectively made by soaking for 24 hours: The extracts were screened for phytochemicals using sample chemical test. Antibacterial and antifungal activities of the sample extract were assayed using agar dilution and Agar diffusing method for zone of inhibition using test organisms, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella sp, Bacterial cultures in nutrient broth and moth extract broth were incubated at 37 °C for 24 hours and were examined for growth. Zones of Inlubitions were examined and measured and results showed that methanolic examined extract was more effective against Klebsiella sp at low concentration of 50 mg/ml compared to aqueous extracts while the ethanolic extract showed high efficacy against all the organism but was more efficient against Klebsiella sp and staphylococcus aureus at same concentration of 25 mg/ml with the highest zone of inhibition 20 mm against Pseudomonas aeruginosa and lowest at 6mm against staphylococcus aureus. Antifungal test using the different extracts on test organism, mucor sp, Rhizopus sp, Aspergillus sp, Candida sp, and Penicillum sp showed that methanolic extract has no effect against other fungi at the highest concentration of 200 mg/ml. The ethanolic extract was more effective against Mucor sp and Candida sp as compared to Rhizopus and Penicillum sp with the zones of inhibition 5.00 mm and 1.00 mm. Based on this Results obtained suggested that Indigofera suffruticosa plant can be used in the treatment of elements caused by the test organisms with particular attention being paid to ethanolic and methanolic extracts of indigofera suffruticosa.
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