ABSTRACT
Probiotics are defined as live microorganisms that confer health benefits when consumed sufficiently. Seven fresh milk samples were randomly collected from lactating cows in Obinze and analyzed following standard microbiological procedures. A total of 10 lactic acid bacteria, including Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus acidophilus, Lactobacillus delbrueckii, Lactobacillus brevis, and Lactococcus lactis were identified. An in vitro activity of LAB isolates against selected enteropathogens (Escherichia. coli, Salmonella spp, and Klebsiella sp) showed a varying degree of inhibition, with Lactobacillus brevis showing the highest inhibition against Escherichia coli (11mm), followed by Lactococcus lactis against Salmonella spp (9mm). Quantitative determination of antimicrobial compounds showed that Lactobacillus plantarum produced the highest quantity of hydrogen peroxide, while Lactobacillus delbrueckii produced the highest amount of lactic acid. This result demonstrated that isolated probiotic bacteria had antimicrobial effects on the test organism. However, these isolates must be characterised to their strain level, and other technological properties investigated before their application as probiotics.
References
[1] Markowiak P, Śliżewska K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 9(2017) 1021.
[2] Salminen S, von Wright A, Morelli L, Marteau P, Brassart D, de Vos WM. Schrezenmeir J. Demonstration of the safety of probiotics—a review. International Journal of Food Microbiology, 83(1) (2004) 41-50.
[3] Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat. Rev. Gastroenterol. Hepatol. 11(2014) 506–514.
[4] Food and Agriculture Organization. Probiotics in food – health and nutritional properties and guidelines for evaluation. Food and Agriculture Organization of the United Nations 2006
[5] De Melo Pereira GV, De Oliveira Coelho B, Magalhães Júnior AI, Thomaz-Soccol V, Soccol CR. How to select a probiotic? A review and update of methods and criteria. Biotechnol. Adv. 36(2018) 2060–2076.
[6] Mokoena MP Lactic acid Bacteria and their Bacteriocins: classification, biosynthesis and applications against Uropathogens. A Mini-review. Molecules, 22(2017) 1255.
[7] Mathur H, Beresford TP, Cotter, PD. Health benefits of lactic acid Bacteria (LAB) Fermentates. Nutrients, 12 (2020) 1679.
[8] Suez J, Zmora N, Segal, E, Elinav E. The pros, cons, and many unknowns of probiotics. Nat. Med. 25(2019) 716–729.
[9] Zoumpopoulou G, Pot B, Tsakalidou E, Papadimitriou K. Dairy probiotics: Beyond the role of promoting gut and immune health, International Dairy Journal, 67(2017) 46-60
[10] De Boeck I, Spacova I, Vanderveken Olivier M, Lebeer S. Lactic acid bacteria as probiotics for the nose? Microbial Biotechnology,14(3) (2021)1751-791
[11] Jiang M, Zhang F, Wan C, Xiong Y, Shah NP, Wei H, Tao X. Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk. J. Dairy Sci. 99 (2016) 1736–1746.
[12] Ito R, Mine Y, Yumisashi Y, Yoshioka R, Hamaoka M, Taji T, Murayama T, Nikawa H. In Vivo Efficacy of Lacticaseibacillus rhamnosus L8020 in a Mouse Model of Oral Candidiasis. J. Fungi (7) (2021) 32
[13] Shin M, Ban OH, Jung YH, Yang J, Kim Y. Genomic characterisation and probiotic potential of Lactobacillus casei IDCC 3451 isolated from infant faeces. Lett. Appl. Microbiol., 2(2021) 578–588
[14] Fiorda FA, De Melo Pereira GV, Thomaz-Soccol V, Rakshit SK, Pagnoncelli MGB, Vandenberghe LPDS. Microbiological, biochemical, and functional aspects of sugary kefir fermentation – A review. Food Microbiol. 66 (2017) 86–95
[15] Plessas S, Nouska C, Karapetsas A, Kazakos S, Alexopoulos A, Mantzourani I. et al. Isolation, characterisation and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from feta-type cheese. Food Chem. 226 (2017) 102–108.
[16] Lebeer S, Vanderleyden J, De Keersmaecker SC. Genes and molecules of lactobacilli supporting probiotic action. Microbiol Mol Biol Rev. 22(4) (2008) 728-64.
[17] Culligan EP, Hill C, Sleator RD. Probiotics and gastrointestinal disease: successes, problems and future prospects. Gut pathogens. 1 (2009) 1–12.
[18] Sanders M E, Akkermans LMA, Haller D, Hammerman C, Heimbach J, Hörmannsperger G, et al. Safety assessment of probiotics for human use Gut Microbes,1(2010) 164–185
[19] Dike KS, Sanni AI. Influence of starter culture fermentation of Lactic Acid Bacteria on the shelf life of Agidi, an indigenously fermented cereal product. African Journal of Biotechnology, 9(46) (2010) 7922-7927
[20] AOAC (Association of Official Analytical Chemists) Official Methods of Analysis, Association of Official Analytical Chemists. 15th Ed. Gaithersburg, USA: AOAC Press; 1990
[21] Edalati E, Saneei B, Alizadeh M, Hosseini SS, Bialvaei A Zahedi, Taheri K. Isolation of probiotic bacteria from raw camel’s milk and their antagonistic effects on two bacteria causing food poisoning, 27(C) (2019) 64-68
[22] Vantsawa PA, Maryah UT, Timothy, B. Isolation and Identification of Lactic Acid Bacteria with Probiotic Potential from Fermented Cow Milk (Nono) in Unguwar Rimi Kaduna State Nigeria. American Journal of Molecular Biology,7(2017) 99–106.
[23] Zhang W, Lai S, Zhou Z, Yang J, Liu H, Zhong Z, Fu H, Ren Z, Shen L, Cao S, Deng L, Peng G. Screening and evaluating lactic acid bacteria with probiotic potential from local Holstein raw milk. Frontiers in Microbiology, 13 (2022) 918774.
[24] Putra TF, Suprapto H, Tjahjaningsih W, Pramono H. The antagonistic activity of lactic acid bacteria isolated from peda, an Indonesian traditional fermented fish IOP Conf. Series: Earth and Environmental Science (2018) 137
[25] Saranya S. Hemashenpagam N. Antagonistic activity and antibiotic sensitivity of Lactic acid bacteria from fermented dairy products Advances in Applied Science Research. 2 (4) (2011) 528-534
[26] Dike KS, Ezekiel CN, Abiala MA, Sanni, AI Antagonistic activity of Lactic Acid Bacteria against Myco-deteriogens of Agidi and Fufu, Two African Fermented Foods. International Journal of Biological Science, 2(9) (2010) 125-134
[27] Prabhurajeshwar C, Chandrakanth RK. Probiotic potential of Lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances. Biomedical Journal,40(5) (2017) 270-283.
[28] Françoise L. Occurrence and role of lactic acid bacteria in seafood products. Food Microbiol. 27(6) (2010) 698-709.
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