Implications of Plant Growth Regulators on Induction of In vitro flowering in Aerva lanata (L.) Juss. ex Schult.

ABSTRACT
In vitro flowering was successfully induced from the shoots regenerated in cultures of Aerva
lanata. In vitro flowering method can be monitored effectively in studying floral transition and flower
development from vegetative to reproductive phase. The nodal shoot segments were used as explants
in the present study. The explants were surface sterilized using HgCl2 and inoculated on Murashige
and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP) for bud break. Maximum
numbers of shoots were induced on MS medium supplemented with 1.0 mg l-1 BAP. The cultures were
multiplied by continuous subculturing of shoots with mother explants on fresh MS medium with BAP
and Kinetin (Kin). Rooting was achieved on one fourth MS medium supplemented with 2.0 mg l-1
indole-3-butyric acid (IBA). The multiplied shoots were subjected to various concentrations of
different plant growth regulators and photoperiod regimes to induce flowering in vitro. The maximum
numbers of in vitro flowers were recorded from the shoots on full strength MS medium with 0.5 mg l-1
BAP and 0.1 mg l-1 indole-3-acetic acid (IAA) on 12/12 h/d photoperiod.

References
[1] C. P. Khare. Springer, New York, 2007.
[2] A. Tushar, Desh mukh, V. Bapuso, S. L. Yadav, S. Badole, L. Bodhankar. Journal of
Applied Biomedicine 6 (2008) 81-87.
[3] S. Sudeesh. Indian Journal of Science and Technology 1 (2012) 7-11.
[4] L. Rajanna, C. Nagaveni, M. Ramakrishnan. International Journal of Botany 7 (2011)
255- 259.
[5] S. Nandagopal, M. Lalitha, S. Abirami, D. Saikrishna, A. Priyan. Der Pharmacia Lettre
7 (2015) 291-298.
[6] A. G. Krishnan, V. K. Ravi, B. C. Nandy, K. C. Meena, S. Dey, P. K. Tyagi, L. K.
Tyagi. International Journal of Phamaceutical Sciences and Drug Research 1 (2009)
191-194.
[7] S. G. Joshi. Oxford and IBH Publishing Co, New Delhi, India, 2007.
[8] T. Vetrichelvan, M. Jegadeesan, P. S. Senthil, N. P. Murali, K. Sasikumar. Indian
Journal of Pharmaceutical Sciences 62 (2000) 300-302.
[9] P. Soundararajan, R. Mahesh, T. Ramesh, V. H. Begum. Indian Journal of
Experimental Biology 44 (2006) 981-986.
[10] S. Venkatesh, J. P. Yanadaiah, N. Zareen, R. B. Madhava. Asian Journal of
Pharmacodynamics and Pharmacokinetics 9 (2009) 58-62.
[11] T. Deepa, R. Vijayalakshmi. International Journal of Current Science 8 (2012) 11-13.
[12] R. Rajesh, K. Chitra, M. P. Padmaa. International Journal of Phamaceutical Sciences
and Drug Research 2 (2010) 269-271.
[13] Y. L. Ramachandra, K. Shilali, A. Mansoor, H. V. Sudeep, B. T Kavitha, H.
Gurumurthy, S. R. Padmalatha. Pharmacologyonline 3 (2011) 435-441.
[14] P. Muthukumaran, P. Shanmuganathan, C. Malathi. Asian Journal of Biochemical and
Pharmaceutical research 1 (2011) 2231-2560.
[15] S. S. Rai, P. Sharma, S. P. Akash. Pharmacologyonline 2 (2011) 921-928.
[16] G. R. Battu, B. M. Kumar. International Journal of Pharmaceutical Science 2 (2012)
74-78.
[17] D. Priya, A. Chaturvedi. Innovative Journal of Medical and Health Science 2 (2012) 44-
46.
[18] B. Tisserat, P. D. Galletta. Horticultural Science 30 (1995) 130-132.
[19] J. Gielis, H. Peeters, K. Gillis, J. Oprins, P. C. Debergh. Proceedings of the twentieth
International Eucarpia Symposium, Melle, edited by H.J. Van and P. Debergh. 2002.
[20] S. M. S. D. Ramanayake, W. A. V. R. Wanniarachchi, T. M. A. Tennakoon. In vitro
Cell Developmental Biology – Plant 37 (2001) 661.
[21] C. S. Lin, C. J. Liang, H. W. Hsaio, M. J. Lin, W. C. Chang. New Forests 34 (2007)
177.
[22] C. S. Lin, C. C. Lin, W. C. Chang. Plant Cell, Tissue and Organ Culture 72 (2003) 71-
78.
[23] M. S. Shekhawat. International Journal of BioScience and Technology 5 (2012) 88-92.
[24] K. S. R. Murthy, R. Kondamudi, C. P. V. Rao, T. Pullaiah. Journal of Agricultural
Technology 8 (2012) 1517-1536.
[25] K. Kanchanapoom, N. Posayapisit, K. Kanchanapoom. Notulae Botanicae Horti
Agrobotanici Cluj-Napoca 37 (2009) 261-263.
[26] M. S. Rathore, N. S. Shekhawat. Agricultural Science Research Journals 1 (2011) 191-
198.
[27] K. Nizam, S. T. Chato. Journal of Agricultural Technology 8 (2012) 1079-1088.
[28] D. Kaur, P. Thapa, M. Sharma, A. Bhattacharya, A. Sood. Indian Journal of
Experimental Biology 52 (2014) 825-834.
[29] T. Pullaiah, V. Ramakrishna, S. Sandya Rani, P. N. Rao. Regency Publications, 2000,
New Delhi, India, p. 243-244.
[30] T. Murashige, F. Skoog. Physiologia Plantarum 15 (1962) 473-497.
[31] C. Heylen, J. C. Vendrig. Plant Cell Physiology 29 (1988) 665-671.
[32] A. Sharma, V. Kumar, P. Giridhar. Electronic Journal of Biotechnology 11 (2008) 1-6.
[33] S. Jana, G. S. Shekhawat. Acta Physiologiae Plantarum 33 (2011) 305-311.
[34] V. Naor, J. Kigel, M. Ziv. Plant Growth Regulation 42 (2004) 7-14.
[35] S. K. M. Haque, B. Ghosh. Environmental and Experimental Biology 11 (2013) 59-68.
[36] J. Kolar, J. Senkova. Journal of Plant Physiology 165 (2008) 1601-1609.
[37] P. Y. E. Q. Luo, Z. Q. Lan. Plant Cell, Tissue and Organ Culture 63 (2000) 73-75.
[38] V. Dielen, V. Lecouvet, S. Dupont, J. M. Kinet. Journal of Experimental Botany 52
(2001) 715-723.
[39] W. L. Lu, S. N. Bai, X. S. Zhang. Acta Botanica Sinica 42 (2000) 996-1002.
[40] C. S. Lin, C. C. Lin, W. C. Chang. Plant Cell, Tissue and Organ Culture 76 (2004) 75-
82.
[41] A. P. Vaz, C. R. Figueiredo, G. B. Kerbauy. Plant Physiology and Biochemistry 42
(2004) 411-415.
[42] K. S. R. Murthy, D. R. Ramulu, J. C. Rao. Phytomorphology 56 (2006) 1-4.
[43] Z. H. Wang, L. Wang, Q. S. Ye. Scientia Horticulturae 122 (2009) 328-331.
[44] K. S. R. Murthy, R. Kondamudi, V. Vijayalakshmi. Journal of Agricultural technology
6 (2010) 179-191.
[45] K. S. R. Murthy, R. Kondamudi. Phytomorphology 60 (2010) 32-37.
[46] J. J. Chavan, M. S. Nimbalkar, A. A. Adsul, S. S. Kamble, N. B. Gaikwad, G. B. Dixit,
[47] R. V. Gurav, V. A. Bapat, S. R. Yadav. Journal of Plant Biochemistry and
Biotechnology 20 (2011) 276–282.
[48] S. Mondal, J. A. T. Silva, P. D. Ghosh. International Journal of Plant Developmental
Biology 5 (2011) 75-77.
[49] J. J. Chavan, A. S. Nalawade, N. B. Gaikwad, R. K. Gurav, G. B. Dixit, S. R. Yadav.
Physiology and Molecular Biology of Plants 20 (2014) 405-410.
[50] M. T. Desai, S. S. Kambale, A. S. Nalwade, N. B. Gaikwad, R. V. Gurav, G. B. Dixit,
S. R. Yadav. Journal of International Academic Research for Multidisciplinary 2
(2014) 124-132.

Download all article in PDF

WSN 32 (2016) 71-81