ABSTRACT
This research was carried out at the Limnology and Water Resources Research Center, National Research and Innovation Agency (BRIN KST Sukarno Bogor). The research was carried out in August-October 2022. This research was intended to determine the optimal light intensity to produce maximum Spirulina platensis biomass, with culture conditions using Zarouk medium modified for trace elements and cultured in a hybrid photobioreactor (combination of flat panel and bubble column) , the light source used is an LED lamp with the light intensity used in this study is 300 μmol/(m2.s) and 200 μmol/(m2.s), the light photoperiod is 24 bright. The parameters studied were Optical Density, biomass concentration, specific growth rate of biomass, biomass productivity, Total Nitrogen (TN) of Biomass and Medium, and Total Phosphate (TP) of Biomass and medium. Cultivation lasted for 11 days, after the data was obtained the Paired T analysis test was used using Microsoft Excel 2019 with a confidence level of 95%. The results showed that the light intensity treatment of 300 μmol/(m 2.s) was the best under conditions of supporting parameters pH ± 9, DO ± 9, and temperature ± 25oC, resulting in an average biomass concentration of 0.208 g/L, an average Optical Density of 0.232, an average rate of specific biomass growth 0.393 divisions/day, average biomass productivity 0.05 g/l/d, the average TN Biomass is 63,508 mg/g, TP Biomass is 8,757 mg/g. Meanwhile, the absorption of TN Medium 0.876 g/L and TP Medium 4.258 g/L showed higher results at a light intensity of 200 μmol/(m 2.s).
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