ABSTRACT
In this research antimicrobial activity of nanoparticles ZnO on perilous bacteria such as
Pseudomonas aeruginosa was evaluated. P. aeruginosa is important pathogen that caused burn wound
infections as it is multi-drug resistant and has several virulence factors. Fifteen samples of P.
aeruginosa were collected from patients who suffering from Burn infections in Al-Hilla teaching
hospital burn unit with the age range between (7-80) years old for both genders. After collecting burn
samples, the diagnosis and characterization were performed by culturing and biochemical tests. ZnO
NPs were synthesized by chemical method, Zinc oxide nanoparticles are well-known to be one of the
multifunctional inorganic compounds which are widely used in medical applications. This study aims
to prepare ZnO nanoparticles with particle size ranging from 23-29  nm. In the present study, surface
modification of ZnO nanoparticles was performed, and influence of modification of the structure and
morphological properties was investigated. The resulting nanoparticles were characterized by X-ray
diffraction (XRD), scanning electron microscopy (SEM) and atomic force (AFM). Zinc oxide
nanoparticles with the average diameter of about 29 nm were modified with an oleic acid to exert
more compatibility. From the results obtained it is suggested that modified ZnO-nanoparticles could
be used effectively in safety environmental and medical applications. Antibacterial activity for
nanoparticle ZnO against P. aeruginosa isolates was measured by: Agar Diffusion Technique and
Minimum Inhibitory Concentration (MIC)/Minimum bactericidal Concentration (MBC) with
microdilution. The best zone of inhibition was (35.5mm) at a concentration of 40 ÎĽg/ml of nano-ZnO
in one strain of P. aeruginosa while the lowest inhibition zone was (16 mm) at a concentration of 20
ÎĽg/ml of nano ZnO in one strain also. In addition, all P. aeruginosa isolates were completely inhibited
at the concentration of 3.7 ÎĽg/ml of nano-ZnO (MIC) but no significant antibacterial activity was
observed at concentrations less than 1.8 ÎĽg/ml of nano-ZnO and the (MBC) was same as MIC (3.7
ÎĽg/ml) for all P.aeruginosa isolates.
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