ABSTRACT
An in vitro regeneration protocol of Russelia equisetiformis was established through nodal shoot
segments cultured on Murashige and Skoog medium (MS) supplemented with different
concentration and combination of cytokinins and auxins in the present study. Cent percent bud
breaking response with maximum number of shoots (6.0) was observed on MS medium augmented
with 1.0 mg/L 6-benzylaminopurine (BAP). Shoots multiplication further enhanced by repeated
subculturing of in vitro shoots on MS medium with 1.0 mg/L BAP + 0.5 mg/L Kinetin (Kn) and
additives. Maximum 13.2 shoots with 7.8 cm length were differentiated. Healthy shoots were rooted
with high frequency (97%) on half strength MS medium containing 1.0 mg/L indole-3 butyric acid
(IBA) with maximum number of roots (5.73). The in vitro regenerated plantlets were hardened in
greenhouse and transferred to nursery with 85% survival rate.
References
[1] E. O. Awe, A. Adeloye, T. Idowu, O. A. Olajide, J. Makinde, Phytomedicine 5 (2008)
301-305.
[2] E. O. Awe, O. S. Banjoko, M. J. Makinde, Inflammopharmacology 18 (2010) 179-185.
[3] E. O. Awe, J. M. Makinde, O. A. Adeloye, S. O. Banjoko, Journal of Natural Products
2 (2009) 3-9.
[4] E. O. Awe, J. M. Makinde, O. A. Olajide, O. K. Wahkeel, Inflammopharmacol 12
(2005) 399-405.
[5] E. O. Awe, J. M. Makinde, Journal of Natural Products 2 (2009) 70-73.
[6] D. Burns, W. F. Reynolds, P. B. Reese, R. G. Enriquez, Magnetic Resonance in
Chemistry 387 (2001) 488-493.
[8] S. Ghatge, S. Kudale, G. Dixit, Asian Journal of Biotechnology 3 (2011) 397-405.
[9] S. Hassan, R. Sultana, M. A. A. Jahan, R. Khatun, Bangladesh Journal of Scientific and
Industrial Research 45 (2010) 95-100.
[10] C. E. Johnson, L. Lin, J. M. Harnly, F.O. Oladeinde, A. M. Kinyua, R. Michelin, Y.
Bronner, Journal of Natural Products 4 (2011) 57-64.
[11] C. E. Johnson, F. O. Oladeinde, A. M. Kinyua, R. Michelin, J. M. Makinde, A. A.
Jaiyesimi, W. N. Mbiti, G. N. Kamau, W. M. Kofi-Tsekpo, S. Pramanik, A. Williams,
A. Kennedy, Y. Bronner, K. Clarke, P. Fofonoff, D. Nemerson, Nigerian Journal of
Natural Products and Medicine 12 (2008) 1-6.
[12] O. T. Kolawole, S. O. Kolawole, Biology and Medicine 2 (2010) 38-41.
[13] O. T. Kolawole, J. M. Makinde, O. A. Olajide, Nigerian Journal of Physiological
Sciences 22 (2007) 59-63.
[14] O. T. Kolawole, O. K. Wakeel, Journal of Medical and Pharmaceutical Sciences 2
(2006) 1-3.
[15] D. Lorke, Archives of Toxicology 54 (1983) 275-87.
[16] R. Mahesh, K. Muthuchelian, M. Maridass, G. Raju, International Journal of Biological
Technology 1 (2010) 111-113.
[17] O. Ojurongbe, J. A. Ojo, D. I. Adefokun, O. O. Abiodun, G. Odewale, O. Awe, Indian
Journal of Pharmaceutical Sciences 77 (2015) 504-510.
[18] O. F. Oladeinde, International Conference, Washington DC, 2005.
[19] A. E. Olorunju, A. Adewale, M. J. Modupe, Journal of Intercultural
Ethnopharmacology 1 (2012) 25-29.
[20] A. K. Patel, M. Phulwaria, M. K. Rai, A. K. Gupta, S. Shekhawat, N. S. Shekhawat,
Scientia Horticulturae 165 (2014) 175-180.
[21] M. Phulwaria, N. S. Shekhawat, J. S. Rathore, R. P. Singh RP, Industrial Crops and
Products 42 (2013) 25-29.
[22] A. V. Raghu, S. P. Geetha, G. Martin, I. Balachandran, K. V. Mohanan, Indian Journal
of Natural Products and Resources 1(2010) 232-235.
[23] K. Ram, N. S. Shekhawat, Physiology and Molecular Biology of Plants 17 (2011) 281-
289.
[24] M. Riaz, N. Rasool, I. H. Bukhari, M. Shahid, A. F. Zahoor, M. A. Gilani, M. Zubair.
African Journal of Microbiology Research 6 (2012) 5700-5707.
[25] M. Saikia, J. H. Pratap, International Journal of Life sciences Biotechnology and
Pharma Research 3 (2014) 85-93.
[26] S. S. Sathish, N. Janakiraman, M. Johnson, Research in Biotechnology 2 (2011) 44-52.
[27] D. P. Sebastian, M. Hariharan, International Journal of Current Microbiology and
Applied Sciences 2 (2013) 56-62.
[28] M. S. Shekhawat, N. Kannan, M. Manokari, C. P. Ravindran, Journal of Genetic
Engineering and Biotechnology (2015b) doi:10.1016/j.jgeb.2015.08.002.
[29] M. S. Shekhawat, N. Kannan, M. Manokari, South African Journal of Botany 100
(2015) 43-50.
[30] M. S. Shekhawat, N. Kannan, M. Manokari, A. Pragasam. The Pharma Innovation 1
(2012) 1-7.
[31] M. Thiyagarajan, P. Venkatachalam, Industrial Crops and Products 37 (2012): 111-
117.
[32] M. Tripathi, N. Kumari, Acta Physiologiae Plantarum (2010) 32: 1011-1015.
[33] J. V. Watkins, T. J. Sheehan, University press of Florida. Florida, 1975, p.6-8.
[34] E. Zayova, M. Petrova, R. Dimitrova, R. Vasilevska, D. Stoeva, Genetics and Plant
Physiology 4 (2014) 155-162
Download all article in PDF
Support the magazine and subscribe to the content
This is premium stuff. Subscribe to read the entire article.
