ABSTRACT
Bioassay guided chromatographic purification of methanolic extract of Pandanus odoratissium
leaves afforded new phenolic compound. The isolated compound was identified as 4-(4-(3,4-
dimethoxyphenyl) hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate by the spectroscope
methods. The puropose of this investigation was to check the effect of the phenolic compound 4-(4-(3,
4-dimethoxyphenyl)hexahyddrofuro[3,4-c]furan-l-yl)-2-methoxyphenyl acetate isolated pure
compound, the human leukemia (HL-60) cell line as model to evaluate the cytoxicity of compound on
the MTT assay. The results obtained from the parameter indicates the compound has significantly
reduced the viability of HL-60 cells shows LD50 values of 21.24 ± 0.5 μg/mL, 10.25 ± 0.3 μg/mL and
5.2 ± 0.6 μg/mL, upon 12, 24 and 48 hours of exposure respective indicates a dose and dependent
response relationship. The present study indicate the compound is highly cytotoxic to human leukemia
(HL-60) cells supports it as an effective therapeutic agent against leukemia
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